Decellularized human umbilical arteries as a potential scaffold for small diameter tissue engineered vascular grafts

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Abstract

Last advances in tissue engineering are connected with the possibility of obtaining decellularized organs and tissues. Their remaining mechanical strength and native structure make them convenient for subsequent recellularization. Decellularization methods vary a lot depending on the kind of the source tissue. We have developed original decellularization method for human umbilical arteries as a potential scaffold for small diameter tissue engineered vascular graft. Effectiveness of decellularization was evaluated by the means of histological and immunogistochemistrical assays, also by estimation of DNA and RNA quantities. These decellularized vessels can be used as scaffolds for cells in frame of tissue engineering vascular grafts making, which demanded in reconstructive cardiovascular surgery.

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About the authors

A. S Nasredinov

V.A. Almazov Federal Heart, Blood and Endocrinology Centre, Saint-Petersburg

A. V Lavreshin

V.A. Almazov Federal Heart, Blood and Endocrinology Centre, Saint-Petersburg

S. V Anisimov

V.A. Almazov Federal Heart, Blood and Endocrinology Centre, Saint-Petersburg

V. N Vavilov

V.A. Almazov Federal Heart, Blood and Endocrinology Centre, Saint-Petersburg

D. I Kurapeev

V.A. Almazov Federal Heart, Blood and Endocrinology Centre, Saint-Petersburg

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