Hyaluronan-based bioplastic material as a scaffold for biomedical cell-based express-product for skin regeneration

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Abstract

This study is designed to investigate the behavior of human fibroblasts and keratinocytes on hyaluronan-based bioplastic material «G-DERM». Cell viability, proliferation, interleikin-6 and keratinocytes growth factor secretion were investigated. The results suggested that «G-DERM» was not toxic and supported cell adhesion and spreading. Histological analysis showed that «G-DERM» properties and porous structure provided distribution of skin cells by cell-type manner. Fibroblasts on «G-DERM», being quiescent, kept their synthetic activity and produced tissue-specific factors of wound healing. According to study results «G-DERM» can be used as 3D cell matrix for regenerative medicine.

About the authors

N. V Kalmykova

A.M. Nikiforov Russian Center of Emergency and Radiation Medicine, Saint-Petersburg, Russia

O. G Spichkina

A.M. Nikiforov Russian Center of Emergency and Radiation Medicine, Saint-Petersburg, Russia

V. N Ellinidi

A.M. Nikiforov Russian Center of Emergency and Radiation Medicine, Saint-Petersburg, Russia

R. R Rakhmatullin

Orenburg State University, Orenburg, Russia

S. I Moiseev

A.M. Nikiforov Russian Center of Emergency and Radiation Medicine, Saint-Petersburg, Russia

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