A novel type of a polytetrafluorethylene-based tissue-engineered construction with nanopattered multifunctional biocompatible nonresorbed coating



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Abstract

Here we preset a method for tissue engineering construct based on highly porous PTFE with nanostructured multifunctional biocompatible non-degradable coating (MBNC) with composition of Ti-Ca-P-C-O-N. It is shown that scaffolds composed of PTFE with MBNC are suitable for adipose-derived multipotent stromal cells CADMSJ active adhesion and proliferation. Under osteogenic stimulation ADMS cells were differentiated to osteogenic lineage, which was confirmed by positive immunohistochemical reaction for osteopontin and osteonectin. Using the experimental model of critical calvarial defects in rabbits we investigated the influence of tissue engineering construct [membrane from highly porous PTFE with MBNC carrying ADMS cells) transplantation on bone defect regeneration. It was shown that under tissue engineering construct the bone defect was completely closed with newly formed bone tissue in 3 and В months after transplantation.

About the authors

A S Grigor'yan

E V Kiseleva s

D V Shtanskiy

M R Filonov

T K Khamraev

A K Toporkova

A V Gastiev

Sh Farkashdi

A S Grigoryan

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

E V Kiseleva

Russian Academy of Sciences [RAS] Koltzov Institute of Developmental Biology, Moscow

Russian Academy of Sciences [RAS] Koltzov Institute of Developmental Biology, Moscow

M P Filonov

The Federal State Educational Institution of the Higher Professional Education «National University of Science and Technology «MISiS», Moscow

The Federal State Educational Institution of the Higher Professional Education «National University of Science and Technology «MISiS», Moscow

D V Shtanskij

The Federal State Educational Institution of the Higher Professional Education «National University of Science and Technology «MISiS», Moscow

The Federal State Educational Institution of the Higher Professional Education «National University of Science and Technology «MISiS», Moscow

Т К Khamraev

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

A K Toporkova

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

A B Gastiev

Russian Medical Academy of Postgraduate Education, Moscow

Russian Medical Academy of Postgraduate Education, Moscow

Sh Farkashdi

Peoples' Friendship University of Russia, Moscow

Peoples' Friendship University of Russia, Moscow

References

  1. Langer R., Vacanti J.P. Tissue engineering. Science 1993; 260(5110): 920-6.
  2. Antonov E.N., Bagratashvili V.N., Whitaker M.J., Barry J.J.A. et al. Three-dimensional bioactive and biodegradable scaffolds fabricated by surface-selective laser sintering. Advanced Mat. 2005; 17(2): 327-30.
  3. Mankani M. H., Kuznetsov S. A., Shannon B. et al. Canine Cranial Reconstruction Using Autologous Bone Marrow Stromal Cells. Am. J. Pat. 2006; 168(2): 547-50.
  4. Zhou S., Greenberger J.S., Epperly M.W. et al. Age-related intrinsic changes in human bone-marrow-derived mesenchymal stem cells and their differentiation to osteoblasts. Aging Cell 2008; 7(3): 335-43.
  5. Киселёва E.B., Васильев А.В. Мультипотентные клетки стромы жировой ткани. В кн.: Биология стволовых клеток и клеточные технологии / под ред. М.А. Пальцева. М.: Медицина 2009; 2: 124- 62.
  6. Zuk Р.А., Zhu М., Ashjian P. et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13(11): 4279-95.
  7. Dudas J.R., Marra K.G., Cooper G.M. et al. The osteogenic potential of adipose-derived stem cells for the repair of rabbit calvarial defects. Ann. Plast. Surg. 2006; 56: 543-8.
  8. Van Harmelen V., Ruling K., Hauner H. Comparison of proliferation and differentiation capacity of human adipocyte precursor cells from the omental and subcutaneous adipose tissue depot of obese subjects. Metabol. 2004; 53(5): 632-7.
  9. Frosch K.H., DrengkA., Krause P. et al. Stem cell-coated titanium mplants for the partial joint resurfacing of the knee. Biomat. 2006; 27(12): 2542-9.
  10. Maeda M., Hirose M., Ohgushi H., Kirita T. In vitro Mineralization by Mesenchymal Stem Cells Cultured on Titanium Scaffolds. J. Biochem. (Jap) 2007; 141(5): 729-36.
  11. Фролова E.H., Мальгинов H.H., Григорьян А.С. и др. Заживление костного дефекта в челюсти кроликов под влиянием ксеногенных мезенхимальных стволовых клеток костного мозга, культивированных на титановых носителях. Рос. стомат. журнал 2008; 3: 12-4.
  12. Левашов Е.А., Штанский Д.В., Глушанкова Н.А., Решетов И.В. Биосовместимые многокомпонентные наноструктурные покрытия для медицины. Патент РФ № 2281122 от 10.08.2006.
  13. Григорьян А.С., Филонов М.Р., Штанский Д.В., Селезнева И.И., Топоркова А.К. Новый тип имплантационного материала на основе политетрафторэтилена с металлическими и керамическими покрытиями. Стоматология 2007; спецвыпуск: 20-26.
  14. Григорьян А.С, Филонов М.Р., Кулаков А.А. и др. Способ получения имплантационного материала на основе пористого политетрафторэтилена и материал, полученный этим способом. Патент РФ №2325191 от 16.02.2007.

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