Current methods in experimental angiogenesis investigation



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Abstract

Growing interest in angiogenesis, a key component in the development of different diseases, requires the use of a suitable experimental model to simulate neovascularization in a laboratory. In recent years, with the development of novel therapeutic strategies, based on angiogenesis regulation, this problem has become especially important. Current in vitro and in vivo models are characterized with a variety of disadvantages, which impede results interpretation. Thus, in vitro assays provide estimation of discrete endothelial cells characteristics, which alter from the same ones in the native microenvironment. The use of in vivo assays is accompanied with difficulties in testing agent delivery and quantitative analysis of its angiogenic activity In view of these complications, the use of a combination of assays is recommended while planning the experiment in this area. The aim of this review is to critically analyze angiogenesis assays, currently used to perform fundamental investigation as well as preclinical tests of developing therapeutic agents

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About the authors

A. A Livanova

S.M. Kirov Medical Military Academy

Email: alexandralivanova@mail.ru

R. V Deev

Human Stem Cells Institute; Kazan (Volga region) Federal University

A. A Rizvanov

Kazan (Volga region) Federal University

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