Comparative analysis of secretory profile of human mesenchymal stromal cells differentiated in myogenic direction



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Abstract

Up to this day there are lots of data accumulated about the role of cytokines in regulation of different tissues homeostasis independently of inflammation framework. Skeletal muscles produce a wide range of biologically active molecules both in a normal condition and after injuries of different etiologies. Moreover, cultures of cells isolated from muscle tissue show same properties. In this regard identification of cytokines profile secreted by cells with myogenic potential is of particular importance as it will help to choose optimal cell types and their sources for clinical application Our research group previously demonstrated the possibility of obtainment of myogenic cells from gingival mucosa derived multipotent mesenchymal stromal cells (MMSC) However, secretory profile of this myogenic cells is not thoroughly investigated to this day The study was conducted on cultures of skin fibroblasts, MMSc derived from the attached and alveolar parts of the gingival mucosa and gingival mucosa MMSc, differentiated in a myogenic direction cells were isolated from skin and gingival mucosa biopsy specimens of 15 healthy volunteers. ELISA assay was performed for evaluation of 48 proinflammatory and anti-inflammatory cytokines, chemokines and growth factors Our data demonstrates tendency of most investigated proteins secretion gradual increase in the following sequence: skin fibroblasts - attached gingival mucosa MMSC - alveolar gingival mucosa MMSC - differentiated myoblasts, including factors directly involved in myogenesis, skeletal muscle homeostasis and regeneration Thus, alveolar gingival mucosa MMSC both before and after induction of myogenic differentiation potentially could facilitate skeletal muscle regeneration Our results indicate that subpopulation of MMSC derived from alveolar gingival mucosa are perspective candidates for clinical usage in patients with skeletal muscle disorders

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About the authors

D. P Samchuk

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

A. A Pulin

Email: andreypulin@gmail.com
Central Clinical Hospital with Outpatient Health Center Moscow, Russia

I. I Eremin

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

I. R Gilmutdinova

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

I. N Korsakov

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

V. L Zorin

Central Clinical Hospital with Outpatient Health Center Human Stem Cells Institute Moscow, Russia

A. I Zorina

Human Stem Cells Institute Moscow, Russia

O. S Grinakovskaya

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

N. L Lazareva

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

P. S Eremin

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

A. P Petrikina

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

A. E Gomzyakov

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

R. V Deev

Human Stem Cells Institute Kazan (Volga region) Federal University Moscow, Russia Kazan, Russia

D. A Timashkov

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

N. K Vit'ko

Central Clinical Hospital with Outpatient Health Center Moscow, Russia

K. V Kotenko

Central Medical Authority of the Business Administration for the President of the Russian Federation Moscow, Russia

P. B Kopnin

N.N. Blokhin Cancer Research Center Moscow, Russia

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