Different expression of hematopoietic-supporting genes in cord, placental and bone marrow mesenchymal stromal cells



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Abstract

Human multipotent mesenchymal stromal cells (MMSC) from bone marrow (BM), umbilical cord (UC) and chorion villi (CV) were isolated and cultured in xeno-free media supplemented with AB human serum There were no differences in expression of CD31, CD33, CD34, CD45, CD90, СD105, CD117, HLA-ABC, HLA-DR between BM, UC and CV MMSC Human AB serum (5%) accelerated proliferation of UC MMSC in vitro. Expression of genes opn, scf, cxcl12, il-3, il-6, il-8, il-11, g-csf, gm-csf, epo, and nes was studied in Real-Time PCR. Up-regulation the expression gene nes in CV MMSC and genes g-csf and il-11 (but 6-fold down-regulation of cxcl12) in UC MMSC, was revealed when compared to BM MMSC (p<0. 03). Expression of il-6 and epo genes in MMCS did not differ. Trend to 10-fold increased expression of gene il-8 as well as cytokine content in UC MMSC vs BM MMCS was demonstrated CV MMSC revealed more differences from the UC MMSC than from the BM MMSC in expression of hematopoietic-supporting genes (opn, scf, il-11, p<0.03). Various abilities of mesenchymal cells from human BM, UC and placenta to express hematopoietic-supporting genes had to be taken into account in application of MMCS for generation of hematopoietic stem cells in vitro

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About the authors

V. S Kostjunina

Republic Research & Production Center for Transfusiology and Medical Biotechnologies

N. V Petyovka

Republic Research & Production Center for Transfusiology and Medical Biotechnologies

Email: npet@blood.by

M. P Potapnev

Belarusian State Medical University

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