Proliferative and differentiation potential of adipose tissue-derived mesenchymal stromal cells in the presence of platelet lysate

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Abstract

Clinical application of mesenchymal stromal cells requires the development of serum-free culture medium for optimal cell growth and differentiation. Platelet lysate as an alternative for xenogeneic fetal serum is able to promote expansion of human adipose tissue-derived mesenchymal stromal cells. Platelet lysate stimulates the proliferative activity of cells and increases the efficiency of colony formation. When cultured mesenchymal stromal cells in the presence of platelet lysate we identified colonies of the same types as in the presence of fetal bovine serum, but their ratio changes in favor of dense and mixed colonies compared with diffuse colonies. Size and density of the cell colonies formed in the presence of platelet lysate are higher than in those in serum. After expansion in medium with 10% platelet lysate mesenchymal stromal cells retain the ability to differentiate into osteogenic and adipogenic directions while efficiency of osteogenic differentiation of these cells is better than of cells previously expanded in the presence of serum. The results of current study show that platelet lysate is a promising natural substitute for xenogeneic serum in optimizing culture technology of human mesenchymal stromal cells for regenerative medicine and tissue engineering.

About the authors

O. Yu Rogulska

Institute for problems of cryobiology and cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine

O. Б Revenko

Institute for problems of cryobiology and cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Yu A. Petrenko

Institute for problems of cryobiology and cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine

A. Yu Petrenko

Institute for problems of cryobiology and cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine

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