Preclinical study of the domestic cord blood stem cell drug "Cryocel"

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Abstract

The article presents the results of studying the effect of the domestic drug Cryocell on the course of ischemic stroke and acute myocardial infarction in an experiment.

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About the authors

K. G. Surkov

All-Russian Scientific Center for the Safety of Biologically Active Substances [VNC BAS]

Author for correspondence.
Email: redaktor@celltranspl.ru

ГУЗ «Городская станция переливания крови»

Russian Federation, Saint-Petersburg

L. A. Belova

All-Russian Scientific Center for the Safety of Biologically Active Substances [VNC BAS]

Email: redaktor@celltranspl.ru

ГУЗ «Городская станция переливания крови»

Russian Federation, Saint-Petersburg

V. K. Krasniakov

All-Russian Scientific Center for the Safety of Biologically Active Substances [VNC BAS]

Email: redaktor@celltranspl.ru

ГУЗ «Городская станция переливания крови»

Russian Federation, Saint-Petersburg

References

  1. Strauer В.Е., Brehm М„ Zeus T. et al. Repair of infarcted myocardium by autologous intracoronary mononuclear bone marrow cell transplantation in humans. Circulation 2002; 106[15): 1913-8.
  2. Chen J., Li Y., Katakowski M. et al. Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat. J. Neurosci. Res. 2003; 73[6): 778-86.
  3. Willing A.E., Vendrame M„ Mallery J. et al. Mobilized peripheral blood cells administered intravenously produce functional recovery in stroke. Cell Transplant. 2003; 12(4): 449-54.
  4. Арзамасцев E.B. и др. Методические указания по экспериментальному изучению препаратов для лечения нарушений мозгового кровообращений. Руководство по экспериментальному [доклиническому) изучению новых фармакологических веществ. М„ 2000: 159-61.
  5. Menzies S.A., Hoff J.T., Betz A.L. Middle cerebral artery occlusion in rats: a neurological and pathological evaluation of a reproducible model. Neurosurgery 1992; 31(1): 100-6.
  6. Borlongan C.V., Hadman M„ Sanberg C.D., Sanberg P.R. Central nervous system entry of peripherally injected umbilical cord blood cells is not required for neuroprotection in stroke. Stroke 2004; 35(10): 2385-9.
  7. Chen J., Sanberg P.R., Li Y. et al. Intravenous administration of human umbilical cord blood reduces behavioral deficits after stroke in rats. Stroke 2001; 32(11): 2682-8.
  8. Vendrame M„ Cassady J., Newcomb J. et al. Infusion of human umbilical cord blood cells in a rat model of stroke dose-dependently rescues behavioral deficits and reduces infarct volume. Stroke 2004; 35(10): 2390-5.
  9. Willing A.E., Lixian J., Milliken M. et al. Intravenous versus intrastriatal cord blood administration in a rodent model of stroke. J. Neurosci. Res. 2003; 73(1): 296-307.
  10. Taguchi A. et al. Administration of CD 34+ cells after stroke enhances neurogenesis via angiogenesis in a mouse model. J. Clin. Invest. 2004; 114:330-8.
  11. Henning R.J. et al. Human umbilical cord blood mononuclear cells for the treatment of acute myocardial infarction. Cell Transplant. 2004; 13: 729-39.
  12. Hirata Y. et al. Human umbilical cord blood cells improve cardiac function after myocardial infarction. Biochem. Biophys. Res. Commun. 2005; 327:609-14.
  13. Yang C. et al. [Transplantation of cord blood endothelial progenitor cells ameliorates limbischemia] Zhonghua Yi Xue Za Zhi 2003; 83:1437-41 [Article in Chinese],
  14. Madeddu P. et al. Transplantation of low dose CD34+KDR+ cells promotes vascular and muscular regeneration in ischemic limbs. FASEB J 2004;18:1737

Supplementary files

Supplementary Files
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1. JATS XML
2. Fig. 1. The effect of the drug on the restoration of coordination in the "rotating rod" test

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3. Fig. 2. The effect of the drug on the reduction of neurological deficit

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4. Fig. 3. The effect of the drug on the reduction of motor asymmetry in the "apo-test"

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5. Fig. 4. The effect of the drug on the dynamics of cognitive function recovery in the URPI test

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6. Fig. 5. The effect of the drug on postinfarction myocardial hypertrophy

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7. Fig. 6. The effect of the drug on exercise tolerance in the "forced swimming" test

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