Our experience in harvesting, testing and storage of hematopoietic cord blood cells

The last two decades in biology and medicine have been marked by an increased interest in the study of human stem cells: their cultivation in vitro, determination of their ability to divide and specialize, application in clinical medicine for the treatment of certain fatal diseases, such as solid tumors, acute and chronic leukemia, aplastic anemia, hereditary metabolic disorders and the immune system, autoimmune diseases, diabetes mellitus, strokes, spinal cord injuries, myocardial pathology and others. The source of stem cells can be bone marrow, stimulated peripheral hematopoietic cells, adipose tissue, epithelial cells of the nasopharyngeal mucosa in the "olfactory epithelium", placental [umbilical] blood, placenta. Obtaining stem cells from the first four sources is somewhat difficult due to the need for surgical intervention and the use of anesthesia. Umbilical cord blood [PC] is collected without risk to the newborn and his mother. Therefore, in the article we want to dwell in detail on one of the stages of preclinical use of placental blood, share our experience in harvesting, laboratory testing and storage of stem cells.