Neural markers in investigation of stem cells differentiation



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Abstract

Investigation of stem cells differentiation substantiates the reasonability of use and confirms clinical effectiveness of new developments in cellular technology. Immunocytochemical methods of revealing of synthesized marker proteins are widely used in evaluation of stem cells differentiation. This review contains characteristics of the most often used markers of neural differentiation that are used in investigation of histoblastic potencies of stem [including neural stem/progenitor) cells. These neuromarkers include nuclear protein of nerve cells NeuN, beta-tubulin III, protein MAP2 associated with microtubules, proteins of neurofilaments, neuron-specific enolase, synaptophysin and molecule of nerve cells adhesion CPSA-NCAM] connected to polysialic acid. The study evaluates the specificity of these markers and reveals the advantages and disadvantages of methods used for their detection. It is emphasized that detection of some proteins expression cannot serve as an unequivocal evidence of stem cells differentiation into neurons. The most neuronspecific markers are: NeuN, neurofilament proteins and synaptophysin.

About the authors

D E Korzhevskiy

E S Petrova

OB Kirik

G V Beznin

E G Sukhorukova

O E Korzhevskii

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

E S Petrova

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

O V Kirik

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

G V Beznin

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

E G Suchorukova

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

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