Uluchshenie dostavki kompleksov plazmidnoy DNKs polikationom v kletki chelovekav prisutstvii bloksopolimerov etilen-i propilenoksida
- Authors: Martynova, AD1, Shevchenko, VD1, Bondar, OV1, Abdullin TI1
-
Affiliations:
- Kazan (Volga Region) Federal University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 101-104
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121628
- DOI: https://doi.org/10.23868/gc121628
- ID: 121628
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Abstract
The work is aimed at the study of the effect of novel
block copolymers of ethylene oxide and propylene oxide
on the delivery of plasmid DNA and its complexes with
cationic polymers into human cells. Tri-functional amphiphilic
block copolymers on the basis of glycerol (LaprolsTM),
polyethyleneimine (25 kDa) and commercial transfection
reagent TurboFectTM were tested as delivery systems.
TurboFect was found to form more compact and positively
charged polyplexes with plasmid DNA (pEGFP-N2) and
provided higher expression of GFP in HEK 293 cells
compared to polyethyleneimine. Laprols weakly interacted
with plasmid DNA and did not improve its intracellular
delivery. However they markedly promoted cell transfection
by DNA-polyethyleneimine complexes. Results show that
Laprols exhibit mild cytotoxicity and produce the interest for
gene therapeutics delivery into cells.
block copolymers of ethylene oxide and propylene oxide
on the delivery of plasmid DNA and its complexes with
cationic polymers into human cells. Tri-functional amphiphilic
block copolymers on the basis of glycerol (LaprolsTM),
polyethyleneimine (25 kDa) and commercial transfection
reagent TurboFectTM were tested as delivery systems.
TurboFect was found to form more compact and positively
charged polyplexes with plasmid DNA (pEGFP-N2) and
provided higher expression of GFP in HEK 293 cells
compared to polyethyleneimine. Laprols weakly interacted
with plasmid DNA and did not improve its intracellular
delivery. However they markedly promoted cell transfection
by DNA-polyethyleneimine complexes. Results show that
Laprols exhibit mild cytotoxicity and produce the interest for
gene therapeutics delivery into cells.
About the authors
A D Martynova,
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
V D Shevchenko,
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
O V Bondar,
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
T I Abdullin
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
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