Human umbilical cord blood mononuclear cells transfected with dual cassette plasmids(vegf + neurotrophic factor) for the treatment of amyotrophic lateral sclerosis
- Authors: Kudryashova NV1, Guseva DS2, Salafutdinov II1, Bashirov FV1, Kiiasov AP1, Rizvanov AA1, Islamov RR2
-
Affiliations:
- Кazan (Volga region) Federal University, Kazan
- Кazan State Medical University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 92-97
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121614
- DOI: https://doi.org/10.23868/gc121614
- ID: 121614
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Abstract
diseases, after neurotraumas and ischemic strokes the
most important neurotrophic and neuroprotective factors,
which can be used as therapeutic agents were identified
in long-term studies in vitro and in vivo. These include
brain-derived neurotrophic factor (BDNF), glial-derived
neurotrophic factor (GDNF), insulin-like growth factor (IGF)
and vascular endothelial growth factor (VEGF). One of the
promising ways of the delivery of supporting neuron survival
factors is considered to be transplantation of genetically
modified cells overexpressing recombinant therapeutic
genes. This article describes generation of cellular delivery
vectors of therapeutic genes - human umbilical cord blood
mononuclear cells genetically modified by dual cassette
plasmids, expressing two therapeutic genes. Efficiency of
transgene expression was confirmed in vitro using RT-PCR.
Analysis of survival, migration, and phenotype of genetically
modified cells was performed 2 weeks after transplantation
into transgenic mice with amyotrophic lateral sclerosis
phenotype.
About the authors
N V Kudryashova
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
D S Guseva
Кazan State Medical University, KazanКazan State Medical University, Kazan
I I Salafutdinov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
F V Bashirov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
A P Kiiasov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
A A Rizvanov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
R R Islamov
Кazan State Medical University, KazanКazan State Medical University, Kazan
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