Changes of angiogenic properties of adipose derived MMSC in patients with coronary heart disease with age

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Abstract

Tissue regeneration is impaired in aged individuals. Adipose- derived multipotent mesenchymal stromal cells (adMMSC), a promising source for cell therapy, were shown to secrete various angiogenic factors and improve vascularization of ischemic tissues. We analyzed how patient age affected angiogenic properties of adMMSC. adMMSC were isolated from surgically obtained subcutaneous fat tissue of patients (n = 64, 43-77 years old) with coronary artery disease (CAD). adMMSC phenotype characterized by flow cytometry was CD90+/ CD73+/CD105+/CD45-/CD31- for all samples and cells were capable for adipogenic and osteogenic differentiation. adMMSC conditioned media stimulated formation of capillary-like tubes by endothelial cells (EA.hy926) and this effect significantly decreased with age of patients. There was no age-associated difference in angiogenic factors gene expression (evaluated by real-time PCR). Level of pro- angiogenic factors in adMMSC conditioned media measured by ELISA significantly declined with age of patients, but level of anti-angiogenic factors did not. Thus, angiogenic properties of adMMSC from aged patients with CAD decline due to the decreasing of pro- angiogenic factors secretion. Our data provide new insights into mechanisms of age-associated impairment of autologous adMMSC therapeutic potential.

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About the authors

A. Y Efimenko

M.V. Lomonosov Moscow State University, Moscow

N. A Dgoyashvili

M.V. Lomonosov Moscow State University, Moscow

N. I Kalinina

M.V. Lomonosov Moscow State University, Moscow

T. N Kochegura

M.V. Lomonosov Moscow State University, Moscow

R. S Achkurin

Russian Cardiology Research and Production Complex of the Ministry of Health, Moscow

V. A Tkachuk

M.V. Lomonosov Moscow State University, Moscow; Russian Cardiology Research and Production Complex of the Ministry of Health, Moscow

E. V Parfenova

M.V. Lomonosov Moscow State University, Moscow; Russian Cardiology Research and Production Complex of the Ministry of Health, Moscow

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