Povyshenie effektivnosti zaseleniya biodegradiruemykh matriksov stromal'nymi i epitelial'nymi kletkami pri dinamicheskom kul'tivirovanii



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Abstract

Adhesion and proliferation of eucariotic cells are charac-terizated by high sensibility to the cultivation conditions, cell mediums, material surface properties, et al. The aim of this study was to improve seeding of stromal and epithelial cells on biodegradable matrices. We tested several polymers perspective for regenerative medicine: polycaprolacton, cellulose diacetate, PLGA, PLA/polycaprolacton. Electrospinning forming membranes had different porousity and fiber sizes. We developed a new method for 3D-matrix seeding with the use of rotation of scaffolds with cells. Based on optimal proliferation activity of the 3T3/NIH and MCF-7 cells we have chosen the scaffold compositions for multilayered cells seeding.

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About the authors

A. V Lyundup

I.M. Sechenov First Moscow State Medical University, Institute for Regenerative Medicine

Moscow, Russia

A. G Demchenko

I.M. Sechenov First Moscow State Medical University, Institute for Regenerative Medicine

Moscow, Russia

T. H Tenchurin

National Research Center “Kurchatov Institute”

Moscow, Russia

M. E Krasheninnikov

I.M. Sechenov First Moscow State Medical University, Institute for Regenerative Medicine

Moscow, Russia

I. D Klabukov

I.M. Sechenov First Moscow State Medical University, Institute for Regenerative Medicine

Moscow, Russia

A. D Shepelev

National Research Center “Kurchatov Institute”

Moscow, Russia

V. G Mamagulashvili

National Research Center “Kurchatov Institute”

Moscow, Russia

R. V Oganesyan

I.M. Sechenov First Moscow State Medical University, Institute for Regenerative Medicine

Moscow, Russia

A. S Orehov

National Research Center “Kurchatov Institute”

Moscow, Russia

S. N Chvalun

National Research Center “Kurchatov Institute”

Moscow, Russia

T. G Dyuzheva

I.M. Sechenov First Moscow State Medical University, Institute for Regenerative Medicine

Moscow, Russia

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