Effect of decellularization with sDs and Triton X-100 detergent solutions on the strength capacities of small caliber arteries

The research is dedicated to evaluation of the effect of the human mammary artery decellularization with 1 % solution of sodium dodecyl sulfate (SDS) and 1% solution of Triton X-100 during 2 hours on its morphological structure and its strength capacities As a result of decellularization all the cellular elements were eliminated from the vessel. Nevertheless, it appeared to be possible to preserve complex spatial structure of the vascular wall. The process of decellularization did not change significantly collagen basis of the vascular wall Although, under 2 hour effect of the detergents one could see lowering collagen concentration down to 80% in intima, media and adventitia. However, even in these circumstances the strength of the vessel was not less than 46% versus that of intact vessels The obtained data are important for the further study aimed at the development of a new generation of vascular implants

tissue engineering, decellularization, decellularized matrices, cardio-vascular surgery