Plasticity of bone marrow-derived stromal cells at grafting onto neural tissue after ischemic injury in vitro



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Abstract

Bone marrow-derived multipotent mesenchymal stromal cells (BM-MMSCs) are able to confer beneficial effects after transplantation into neural tissue with ischemic injury. This effect is probably caused by the release of trophic factors, although the possibilities of replacement of dead neural cells by BM-MMSCs are not excluded. The aim of this study was to identify the ability of BM-MMScs to differentiate into cells of the nervous tissues and their neuroprotective effect in direct contact with nervous tissue damaged by ischemia Therefore, we investigated this interaction by in vitro model of organotypic hippocampal tissue to avoid affecting the immunological processes in the conditions after transplantation in vivo. Ischemic injury induced by oxygen-glucose deprivation The potential of differentiation of transplanted multipotent mesenchymal bone marrow stromal cells to neural direction was assessed for 14 days after the ischemic injury. At the 7 th day after the oxygen-glucose deprivation and transplantation the multipotent mesenchymal bone marrow stromal cells differentiated into microglial cells, and on the 14th day - as in microglial cells and in mature oligodendrocytes These findings suggest that the transplanted stem cells respond to signals from the microenvironment of the injured tissue of the recipient, which in turn may trigger and regulate cell differentiation as well as to determine the direction of migration

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About the authors

O. A Rybachuk

Bogomoletz Institute of Physiology NAN Ukraine; State Key Laboratory of Molecular and Cell Biology; State Institute of Genetic and Regenerative Medicine NAMS Ukraine

Email: oks-ribachuk@yandex.ru

V. M Kyryk

State Institute of Genetic and Regenerative Medicine NAMS Ukraine

P. A Poberezhnyi

State Institute of Genetic and Regenerative Medicine NAMS Ukraine

G. M Butenko

State Institute of Genetic and Regenerative Medicine NAMS Ukraine

T. A Pivneva

Bogomoletz Institute of Physiology NAN Ukraine; State Key Laboratory of Molecular and Cell Biology; State Institute of Genetic and Regenerative Medicine NAMS Ukraine

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