


Vol 8, No 3 (2013)
- Year: 2013
- Articles: 77
- URL: https://genescells.ru/2313-1829/issue/view/6113
-
Description:
VI Annual International Symposium
"Actual issues of gene and cell technologies"
Moscow, October 11-12, 2013
Articles
Immunomodulatory effect of mesenchymal stem cells on allergen activated lymphocytes
Genes & Cells. 2013;8(3):6-6



Increasing engraftment and survival of full-thickness skin grafts with use of stromal-vascular fraction derived from adipose tissue
Genes & Cells. 2013;8(3):6-7



The experience of clinical use of allogeneic MSCs in the early postoperative period in dogs with neurological disorders secondary spinal cord compression in the thoraco-lumbar region
Genes & Cells. 2013;8(3):7-8



The influence mesenchymal stromal cells adipose tissue on development of rats' experimental glioma
Genes & Cells. 2013;8(3):8-9



Development of «activated» osteoplastic material containing plasmid DNA with vegf-a165 gene
Genes & Cells. 2013;8(3):9-10



Experimental partial and complete transection spinal cord and its bioengineering reconstruction
Genes & Cells. 2013;8(3):10-11



Transcriptome analysis of human multipotential mesenchymal stromal cells' response to nonionic block copolymers
Genes & Cells. 2013;8(3):11-12



Algorithm for estimating the safety of autologous cell cultures for clinical trials
Genes & Cells. 2013;8(3):12



Human endometrial stromal cells: in vitro biological properties under conventional and low oxygen cell culture conditions
Genes & Cells. 2013;8(3):13



Neural crest - derived multipotent stem/progenitor cells in culture in vitro: self-renewal, clonal multipotency and spherogenesis
Genes & Cells. 2013;8(3):14



Detection of apoptosis pathway's molecular lesions in acute megacarioblastic leukemia by RNA-seq
Genes & Cells. 2013;8(3):15-16



MPROVING THE TECHNOLOGY OF INFLUENZA VACCINE
Genes & Cells. 2013;8(3):16



Detection of mycoplasma contamination in mammalian cell cultures
Genes & Cells. 2013;8(3):16-17



Application of stem cells for activation of hemopoiesis in old laboratory animals in the conditions of acute hemorrhage
Genes & Cells. 2013;8(3):17



Possibilities of differentiation of native and in vivo activated hepatic stellate cells transplanted into rats after partial hepatectomy or liver damage by carbon tetrachloride
Genes & Cells. 2013;8(3):17-18



Elimination of colorectal cancer stem cells in vivo via suicidal technology
Genes & Cells. 2013;8(3):18-19



Increase the volume of bone tissue in dental implantation using osteoplastic materials
Genes & Cells. 2013;8(3):19-20



Bone marrow brain death-donor as a potential source for allogenic cell products
Genes & Cells. 2013;8(3):20



The assessment of morphofunctional properties of skin fibroblasts after ionizing irradiation
Genes & Cells. 2013;8(3):21



Treatment of full breaks forward superficial flexor in horses with use of multipotent mesenchymal stromal cells derived from adipose tissue
Genes & Cells. 2013;8(3):22



Development of an experimental model of treatment of liver fibrosis with stromal-vascular fraction of adipose tissue
Genes & Cells. 2013;8(3):22-23



Age-associated activation of urokinase system and MMPs in adipose-derived multipotent mesenchymal stromal cells from patients with coronary artery disease (CAD)
Genes & Cells. 2013;8(3):23-24



Concentration of nuclear heterochromatine in buccal epithelium cells in groups of patients with various degree of diseases as the bioage index
Genes & Cells. 2013;8(3):24-25



The study of phenotypic profile and osteogenic properties of gingival fibroblasts
Genes & Cells. 2013;8(3):25-26



Comparative analysis of serums and mediums for fibroblasts cultivation
Genes & Cells. 2013;8(3):26-28



The study a possibility to use multipotent mesenchymal stromal cells isolated from human gingiva in tissue-engineering construction for periodontal bone repair
Genes & Cells. 2013;8(3):28-29



Medical cell technologies in esthetic medicine and reconstructive surgery: opportunities and prospects
Genes & Cells. 2013;8(3):29-30



Modeling of retinal function compensation in retinal dystrophies during stem cell treatment
Genes & Cells. 2013;8(3):30-31



Influence stromal-vascularis fractions of cells of a bone brain on healing of skin wounds at mice
Genes & Cells. 2013;8(3):31



Experimental justification for the use of cellular technologyfor the treatment of inflammatory-destructive diseases of joints
Genes & Cells. 2013;8(3):31-32



The comparative analysis of the methods for evaluation of proliferative activity of rats' bone marrow mononuclears on the background of allogeneic mononuclear cells transfusion
Genes & Cells. 2013;8(3):33



PGD: a rational reproductive choice for carriers of single-gene disorders
Genes & Cells. 2013;8(3):33-34



Transplantation of multipotent mesenchymal stromal cells after extensive resection of the liver on rats model
Genes & Cells. 2013;8(3):34-35



Innovative minimally invasive administration of adipose derived regeneratory cells for the treatment of urethral strictures
Genes & Cells. 2013;8(3):35-36



Transplantation of allo-and xenogeneic endocrine tissues into immune privileged areas of the body
Genes & Cells. 2013;8(3):36



Different functional activity of multipotential mesenchymal stromal cells and their precursses
Genes & Cells. 2013;8(3):36-37



Study of biological properties of stromal vascular fraction cells isolated from adipose tissue
Genes & Cells. 2013;8(3):37-38



Porous ceramics based on ZrO2 or Al2O3: physical properties and preliminary evaluation of biocompatibility
Genes & Cells. 2013;8(3):38-39



Newborns genetic screening results for the most frequent autosomal recessive hereditary disorders
Genes & Cells. 2013;8(3):39-40



Immunogenic properties of mice foetal neurocells
Genes & Cells. 2013;8(3):41



Study of the neurogenetic differentiation of foetal brain cells in vitro
Genes & Cells. 2013;8(3):42



Development of tissue engineering constract based on nikelid titanium and mesenchymal stromal cells of gingival for correction of bone defects in maxillofacial region
Genes & Cells. 2013;8(3):43



Using the induction of hematopoietic stem cells for correction the bioage
Genes & Cells. 2013;8(3):43/1



The osteogenic effect of bone morphogenetic protein-2 on collagen scaffold with fibronectin
Genes & Cells. 2013;8(3):44



Morpologycal aspects of the cultivation of limbal cells on the amnion
Genes & Cells. 2013;8(3):45-46



Sibling cord blood banks based on the model of personal storage of umbilical cord blood
Genes & Cells. 2013;8(3):46-47



Morphofunctional properties of human adult adipose tissue multipotent mesenchymal stromal cells during culture in the presence of platelet lysate
Genes & Cells. 2013;8(3):47-50



Chitosan and polylactide scaffolds biocompatibility characterisation for human mesenchymal stem cells
Genes & Cells. 2013;8(3):50



Experimental study of the xenogenic origin biological membranes
Genes & Cells. 2013;8(3):51



Use of the chemiluminescent analysis in made dendritic vaccine
Genes & Cells. 2013;8(3):51-52



B-1 INTEGRIN AS A POTENTIAL THERAPEUTIC TARGET FOR SECONDARY CATARACT
Genes & Cells. 2013;8(3):52-53



Genetic modification of human adipose derived stem cells with pEGFP-N2 plasmid DNA does not affect cytokines/chemokines secretion
Genes & Cells. 2013;8(3):53-54



Influence of cleaning cryoprotectant DMSO of material for transplatation on properties of hematopoietic stem cells
Genes & Cells. 2013;8(3):54



Modeling of interneuronal syncytial connection in vitro
Genes & Cells. 2013;8(3):54-55



Algorithm obtaining of the hematopoietic stem cells peripheral blood for autologous transplantation
Genes & Cells. 2013;8(3):55-56



The use of autologous dermal fibroblasts in cosmetology
Genes & Cells. 2013;8(3):56-57



Immunogenicity of plasmid vector co-expressing recombinant proteins using foot-and-mouth disease virus 2A-peptides
Genes & Cells. 2013;8(3):57-58



Generation of cell model system of amyotrophic lateral sclerosis based on patient-specific induced pluripotent stem cells
Genes & Cells. 2013;8(3):58



The prevention opportunities of inheritance of monogenic diseases from reproductive cells donors
Genes & Cells. 2013;8(3):58-59



The eperience in development and implementation of hereditary diseases genetic diagnosis based on DNA microarrays
Genes & Cells. 2013;8(3):59



Study of the influence of stem cells for the regeneration of the spleen in the conditions of ionizing radiation
Genes & Cells. 2013;8(3):60



Efficiency of autofibroblasts in surgical treatment of parodontitis
Abstract
Dysferlinopathies is a group of autosomal-recessive inherited neuromuscular diseases, which are characterized by defect in mRNA expression or in functionioning of dysferlin protein, appearing in about 1/200 000 births. Dysferlin is encoded by DYSF gene (Dystrophy-associated fer-1-like). It's disruption can cause various types of primary dysferlinopathies, which include Miyoshi myopathy (MM), Limb-girdle Muscular Dystrophy type 2B (LGMD2B) and distal myopathy with anterior tibial onset. Also, dysferlin deficiency can be associated with other diseases, such as caveolin- and calpainopathies. Here we discuss dysferlin protein structure and function, it's clinical phenotypes, known animal models and developing treatment strategies for dysferlinopathies.
Genes & Cells. 2013;8(3):61-70



Efficiency of autofibroblasts in surgical treatment of parodontitis
Abstract
The destructive forms of periodontal disease have a high prevalence. Our study was aimed to evaluate the effectiveness of tissue-engineering osteoplastic material in treatment of patients with periodontal pathology. We have obtained autologous gingival fibroblasts of patients, combined with the hydroxyapatite carriage and transplanted on a standard surgical treatment of periodontitis destructive forms. As a control we used the material without cells implanted into periodontal defects of other affected teeth of the same patients. We have shown the better effect of tissue-engineering osteoplastic material regarding the periodontal pockets depth, but significant difference in bone regeneration and other indicators has not been revealed. Thus, a continuation of researches is required to modify the technology of tissueengineering osteoplastic material creation and to choice the more optimal carrier for gingival fibroblasts.
Genes & Cells. 2013;8(3):72-77



Construction and biological effect evaluation of gene-activated osteoplastic material with human vegf gene
Abstract
Development of new effective osteoplastic materials is highly requested in practice of traumatology and orthopedics, oral and maxillofacial surgery. The goals of our research were design and construction of gene-activated bone graft (GABG) consisting of collagen/hydroxyapatite scaffold and plasmid DNA encoding vegf-a165 and evaluation of its biological effect in vitro and in vivo. We have shown that GABG co-incubation with multipotent mesenchymal stromal cells increased their VEGF protein expression. After GABG implantation into parietal bones defects the transfection of «recipient bed cells» was observed and accompanied by more pronounced angiogenesis as compared with control. The lager volume of bone regenerate was in case of GABG on 15 and 30 days after application. The source of reparative osteogenesis was not only parietal bones but also the GABG fragments (even from central part of the defect) majority of which were surrounded by newly formed bone tissue. In control group no osteoinductive effect has been observed. Thus, GABG with plasmid DNA encoding VEGF-A165 possesses angiogenic activity providing osteoinductive properties.
Genes & Cells. 2013;8(3):78-85



Decellularized rat heart matrix as a basis for creation of tissue engineered heart
Abstract
Development of bioengineered scaffolds of internal organs is one of the priority areas of tissue engineering. Decellularization allows to obtain biological (natural) scaffolds while preserving extracellular matrix and three-dimensional structure of organs. The primary goals of the present research were to investigate pathological characteristics of the decellularized rat heart scaffold and evaluate adhesion and viability of multipotent mesenchymal stromal cells (MMSC) during recellularization. Rat hearts were decellularized using a modified detergent-enzymatic method including sodium deoxycholate and DNAse. The results of morphological studies have confirmed the safety of extracellular matrix proteins and patency of the coronary vessels. Mechanical testing of decellularized and native samples of rat heart showed an increase of mechanical properties of the matrix during decellularization. During the conducted experiments on recellularization of obtained scaffold has been shown that the extracellular matrix was not toxic for MMSC which were viable and maintained their metabolic activity during prolonged cultivation on the scaffold.
Genes & Cells. 2013;8(3):86-94



Possible directions of human cord blood mononuclear cells differentiation in the regenerating rat liver
Abstract
It is known that human cord blood hematopoietic stem cells (HSC) are able to differentiate into hepatocytes. This ability can be widely used in treatment of various liver diseases. However, there are some genetic diseases of liver, when the application of autologous stem cells is not possible. So it could be very helpful to develop methods of genetic modification of stem/progenitor cells. However, it should be proved that genetic modification does not change the properties of HSC. We performed partial hepatectomy for the white mongrel male rats and injected human umbilical cord blood mononuclear cells transfected by gene of green fluorescent protein (GFP) into the spleen. Paraffin sections of the liver were stained with antibodies to stem cell factor receptor, human leukocyte antigen, a-smooth muscle actin, enhanced GFP, cytokeratin 19, hepatocyte specific antigen, human a-fetoprotein. Also we used a double-immunohistochemical staining to detect expression of stem cell factor receptor and desmin, enhanced GFP and cytokeratin 19. Our study showed that human cord blood mononuclear cells transfected by gfp transplanted into the spleen of rats after partial hepatectomy migrated to the liver and acquired the phenotype of hepatocytes, cholangiocytes and sinusoidal cells. At the same time the differentiation of such transplanted cells into myofibroblasts, as it was previously shown, does not occur. Hepatoblasts and hepatocytes found in the liver of rats after transplantation of genetically modified and native cells express human hepatocyte specific antigen and a-fetoprotein that means they are functionally active.
Genes & Cells. 2013;8(3):95-100



Changes of liver microstructure after partial hepatectomy in rats
Abstract
Ability of mammalian liver to regenerate is one of the favorite examples of “regenerative medicine”. At the same time liver regeneration can not be viewed as a simple hypertrophy, it must have some appropriate steps. Understanding of these processes is crucial for correct interpretation of liver therapy results, especially after cellular therapy. But, unfortunately, original and first-hand data regarding changes in liver microstructure during regeneration is relatively scarce. This work was dedicated to study changes of liver microstructure during liver regeneration after partial hepatectomy in rats. We analyzed proliferative processes, perisinusoidal cells involvement, sizes of classical hepatic lobules, participation of bile ducts, branches of afferent and efferent hepatic vessels in liver regeneration on 1, 2, 3, 5, 7 postoperative days. Our results have shown that liver microstructure during regeneration after partial hepatectomy undergoes two stages: hypertrophy of hepatic lobules by proliferation of liver cells until 4th day and division of hepatic lobules by branching of bile ducts, hepatic artery, portal and central veins from 4th until 7th postoperative day.
Genes & Cells. 2013;8(3):101-105



Genetically modified human umbilical cord blood mononuclear cells as potential stimulators of neuroregeneration in degenerative disorders of central nervous system
Abstract
Gene-cell therapy is a new step for the treatment of different human disorders including central nervous system degenerative diseases. In this review we focused on the last challenges in the field of human umbilical cord blood mononuclear cells transplantation - an attempt to support neuronal cells survival and to stimulate the neuroregeneration. As a potential therapy for the treatment of neurodegenerative diseases we reviewed the latest advances in gene modification of human umbilical cord blood mononuclear cells as a novel tool for the effective delivery of neuroprotective factors and growth factors in the injured or degenerative areas of the central nervous system under pathological conditions. The main topic of this review is the potential therapy of the amyotrophic lateral sclerosis - the progressive neurodegenerative disorder affecting primarily upper and lower motoneurons - by using genetically modified human umbilical cord blood mononuclear cells. The results from the up-to-date experiments indicated the opportunity to obtain differentiated macrophages, endothelial cells, or astrocytes from the genetically modified human umbilical cord blood mononuclear cells after their transplantation in the mouse model of the amyotrophic lateral sclerosis. Taken together, these data build the high-capacity platform for the supporting of degenerating neurons, structural and functional recovery of the brain and spinal cord after trauma, ischemia and other neurodegenerative disorders.
Genes & Cells. 2013;8(3):106-112



C-kit and desmin-positive cells in expression in islets of pancreas during alloxan diabetes in rats
Abstract
Nowadays there are findings that C-kit-positive and desmin-positive stellate cells help to regenerate endocrine part of pancreas. But still it is unknown about their role and the way of differentiation of these cells during pancreas regeneration. That's why the aim of our work was to study the dynamic of these cells population during alloxan diabetes in rats. The work was made on 33 rats with experimental diabetes. Blood glucose, insulin and glucagon levels were analyzed. Also the expression of desmin (marker of stellate cells), a-SMA (marker of myofibroblast), C-kit (marker of endocrine stem cells), insulin and glucagon (marker of differentiated a- and р-cells of Langerhans islets) was studied. The expression of desmin was found after one day of experimental diabetes in islets cells of pancreas. Maximum of these cells was after the third day of the experiment. Also after one day of the experiment C-kit-positive cells, which expressed insulin and glucagon were found. We suppose that stellate cells are the main factor of microenvironment for differentiation of C-kit-positive progenitor cells into р-cells through the stage of glucagon-positive cells because stellate pancreas cells can produce different growth factors and components of intercellular matrics.
Genes & Cells. 2013;8(3):113-115



Use of stromal vascular fraction of adipose tissue for treatment of femur pseudoarthtosis: case report
Abstract
The article presents a case report of successful treatment of pseudarthrosis of the femur with the use of autologous cells of the stromal vascular fraction (SVF) derived from adipose tissue. Autologous SVF cells mixed with fibrin glue were transplanted into bone defect by injection under the control of electro-optical converter. Two months after injection, the patient reported the disappearance of pain. After 4 months we observed the recovery of function of the knee, X-ray shows signs of the fracture healing through the formation of bone callus.
Genes & Cells. 2013;8(3):116-118



Characterization of rat myofibroblasts isolated from liver portal tracts using explantation technique
Abstract
Today there is no effective approach to treat liver fibrosis and the only way is transplantation of donors' liver. Investigation of molecular-cellular mechanisms of liver fibrosis can help to discover new ways of slowing down or even reverse the process of fibrogenesis in liver. For a long time hepatic stellate cells were undeservingly blamed for being the major causer of liver fibrosis, because they were considered as the main source of myofibroblasts, that synthesize connective tissue extracellular matrix. In this particular work explantation approach was used to isolate cell culture from portal tracts. It was shown that received cells are portal fibroblasts, and, just as hepatic stellate cells, in case of liver alteration can differentiate in myofibroblasts, that express а-smooth muscle actin. During long-term cultivation it was shown that portal myofibroblasts can differentiate into fibroblasts and back on late passages. So, we can conclude, that there is a potency to reverse the process of fibrogenesis in liver.
Genes & Cells. 2013;8(3):119-124



Combined treatment of throphic ulcer of the heel using vacuum therapy with direct gene therapy: case report
Abstract
In this article we describe a clinical case of successful treatment of neurotrophic ulcers of heel region using classical surgical techniques such as debridement and autodermoplastics combined with vacuum therapy and injection of double cassette expression plasmid encoding cDNAs of vascular endothelial growth factor and fibroblast growth factor 2. The outcome of therapy opens new horizons in the treatment of such diseases in short period of time.
Genes & Cells. 2013;8(3):125-128



Delivery of cord blood cells modified with adenoviral vectors expressing GDNF into the area of spinal cord injury stimulates recovery of motor function and supports a population of glial cells
Abstract
On the model of rat spinal cord dosed contusion at Th8 level studied the effect of delivery into the area of damage of glialcell-line-derived neurotrophic factor gene using adenovirus-transduced of human umbilical cord blood mononuclear cells on motor recovery and maintaining a population of glial cells. The results show that the proposed method of gene-cell therapy can effectively stimulate the regeneration of posttraumatic spinal cord injury, which is manifested in the form of improved indicators of recovery of motor function, increasing the number of reactive astrocytes and oligodendrocytes progenitors.
Genes & Cells. 2013;8(3):129-132



Long-term results of autologous peripheral blood hematopoietic stem cell transplantation in patients with peripheral arterial diseases
Abstract
The aim of our work was to assess long-term results of autologous peripheral blood hematopoietic stem cell application in patients with peripheral arterial diseases. Peripheral blood hematopoietic stem cells mobilized by granulocyte colony-stimulating factor were transplanted intramuscularly to 30 patients with peripheral arterial diseases (IIb stage by Pokrovsky). Standart tredmill test, ankle-brachial index estimation and ankle-brachial index restoration time estimation after loading were performed on 0, 3, 6, 12 and 60 months after transplantation. Immunohistochemical study of injured gastrocnemius muscle biopsies taken before peripheral blood hematopoietic stem cells transplantation and 3 months after the procedure was performed. Peripheral blood hematopoietic stem cells transplantation increases capillary density (22,4%, p = 0,0005). Ankle- brachial index increased by 18,1% on month 6 after transplantation without a tendency to change on month 12. 60 months after transplantation initial to hematopoietic stem cells transplantation ankle-brachial index rates were marked. Painless walking distance was increasing at all times of observation progressively, on month 60 no walking distance limitation was marked by most patients. Ankle-brachial index restoring time shows positive trend of the functional state of limb during the first year after transplantation, 60 months after transplantation it showed no «walking reserve» limitation in most patients. 5-years survival was 79%, death causes were stroke, cardiac pathology (3 cases), lung cancer. So, peripheral blood hematopoietic stem cells transplantation allows eliminating peripheral arterial diseases symptoms and preserving limb in long-term period. Autologous transplantation of peripheral blood hematopoietic stem cells has no complications and is safe for therapy of patients with peripheral arterial diseases II stage.
Genes & Cells. 2013;8(3):133-136



Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction
Abstract
Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3x106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against C-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and а-smooth muscle actin (a-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of a-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on C-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis
Genes & Cells. 2013;8(3):137-141



Generation of recombinant adenoviral vectors encoding neural cell adhesion molecules ncam1, ncam2 and l1cam
Abstract
To succed in gene therapy it is necessary to observe few basic conditions, such as targeted delivery of the therapeutic gene into the target organ and its effective expression. Nowadays targeted delivery of therapeutic genes is one of the critical problems of gene therapy. Delivery of recombinant genes using cell carriers (vectors), expressing tissue-specific cell adhesion molecules, allows us to move toward solving this problem. Using Gateway cloning technology (Invitrogen) we have created recombinant expression constructs pAd-NCAM1, pAd-NCAM2 and pAd-L1CAM, encoding neural cell adhesion molecules. Expression of recombinant proteins has been confirmed by immunofluorescent analysis. Based on these genetic constructs recombinant adenoviruses (serotype 5) were generated and titered. Obtained viral vectors encoding neural cell adhesion molecules may be subsequently used to modify cells carrying additional therapeutic genes to increase the efficiency of gene-cell therapeutics delivery to target organ.
Genes & Cells. 2013;8(3):142-146



Comparison of different methods of rat hepatic stellate cells isolation, labeling and transplantation
Abstract
Nowadays more and more attention is turned to hepatic stellate cells (HSC) and their role in liver regeneration. Nonetheless there are several methodological questions, for example, methods of HSC isolation, their labeling and ways of transplantation. In this work we compared two different methods of HSC isolation: collagenase-pronase liver perfusion with further Histodenz density gradient centrifugation and method of Seglen for isolation of hepatocytes associated with HSC. We also analyzed diverse methods of cells labeling: membrane fluorescent labels PKH26 and with gene of green fluorescent protein (GFP), that could be get into the cells by electroporation, with chemicals like TurboFect or by adenovirus. Then cells were transplanted into rats in two ways: into lien and into system of portal vein. According to our results, we able to conclude that collagenase-pronase liver perfusion with further cells gradient centrifugation in Histodenz is better for HSC isolation than method of Seglen, the most optimal method for cells labeling is with adenovirus, expressing the GFP gene, for HSC transplantation - transplantation into system of portal vein.
Genes & Cells. 2013;8(3):147-151


