Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute63292610.17816/gc632926Original Study ArticlesОригинальные исследованияResearch ArticleEvaluation of chondrogenic potential of human dermal fibroblasts after modification with differentiation media and cytokine TGF-β3Оценка хондрогенного потенциала дермальных фибробластов человека после модификации дифференцировочными средами и цитокином TGF-β3https://orcid.org/0000-0002-9931-33944843-4807BozhokinMikhail S.БожокинМихаил Сергеевич
Russian Federation

Cand. Sci. (Biology)

канд. биол. наук

writeback@mail.ruhttps://orcid.org/0000-0002-7940-94447318-5450MarchenkoDaria M.МарченкоДарья Максимовна
Russian Federation
berbimot@yandex.ruhttps://orcid.org/0000-0001-8576-37171056-7878MikhaylovaElena R.МихайловаЕлена Радиславовна
Russian Federation

Cand. Sci. (Biology)

канд. биол. наук

mikhailovaer@yandex.ruhttps://orcid.org/0009-0003-5807-5750RakhimovBulat R.РахимовБулат Раисович
Russian Federation
rah.bulat7@yandex.ruhttps://orcid.org/0000-0002-2083-24243086-3694BozhkovaSvetlana A.БожковаСветлана Анатольевна
Russian Federation

MD, Dr. Sci. (Medicine)

д-р мед. наук

clinpharm-rniito@yandex.ruhttps://orcid.org/0000-0002-8080-904X5169-7740KornevaYulia S.КорневаЮлия Сергеевна
Russian Federation

MD, Cand. Sci. (Medicine)

канд. мед. наук

ksu1546@yandex.ruhttps://orcid.org/0000-0002-9104-83032462-1939AleksandrovaSvetlana A.АлександроваСветлана Алексеевна
Russian Federation

Cand. Sci. (Biology)

канд. биол. наук

alekssvet2205@gmail.comhttps://orcid.org/0000-0002-8293-63688924-6418KhotinMikhail G.ХотинМихаил Георгиевич
Russian Federation

Cand. Sci. (Biology)

канд. биол. наук

h_mg@mail.ruInstitute of Cytology of the Russian Academy of SciencesИнститут цитологии Российской академии наукVreden National Medical Research Center of Traumatology and OrthopedicsНациональный медицинский исследовательский центр травматологии и ортопедии им. Р.Р. ВреденаSaint-Petersburg State Institute of TechnologyСанкт-Петербургский государственный технологический институт (технический университет)North-Western State Medical University named after I.I. MechnikovСеверо-Западный государственный медицинский университет им. И.И. Мечникова03092024200920241933723862905202414082024Copyright ©; 2024, Eco-VectorCopyright ©; 2024, Эко-Вектор2024Eco-VectorЭко-Векторhttps://creativecommons.org/licenses/by-nc-nd/4.0/https://genescells.ru/2313-1829/article/view/632926

BACKGROUND: Hyaline cartilage is an avascular tissue that envelopes the surface of major joints. The regenerative capacity of this tissue is restricted due to its high content of extracellular matrix proteins and its modest number of cells. Articular cartilage recovery remains relevant and yet unresolved to date. Utilizing biomedical products that contain modified cells is a promising approach.

AIM: To examine the effects of various culture media on the chondrogenic modification of cells and to compare the results.

MATERIALS AND METHODS: Human dermal fibroblasts and rabbit multipotent mesenchymal stromal cells were modified using the chondrogenic differentiation medium StemPro or recombinant TGF-β3 protein. Alterations in the expression of genes involved in chondrogenesis (Acan, Tgfβ3, Col2α1, Comp) were assessed using the real-time polymerase chain reaction method ΔΔCt.

RESULTS: It was demonstrated that human dermal fibroblasts can induce chondrogenic modification when used in protein media. This cell type is easy to harvest, does not necessitate special cultivation conditions, is readily scalable, and is suitable for allogeneic transplantation.

CONCLUSION: The obtained data can be employed to develop tissue engineering products for the regeneration of hyaline cartilage using allogeneic dermal fibroblasts.

Обоснование. Гиалиновый хрящ представляет собой аваскулярную ткань, покрывающую крупные суставы. Данная ткань с малым количеством клеток и большим содержанием белков внеклеточного матрикса обладает ограниченной способностью к регенерации. Восстановление поверхности суставного хряща на сегодняшний момент является актуальной и не до конца решённой задачей. Перспективным направлением можно считать применение биомедицинских продуктов, содержащих модифицированные клетки.

Цель исследования — выявление влияния различных питательных сред на хондрогенную модификацию клеток и сравнение результатов между собой.

Материалы и методы. Дермальные фибробласты человека и мультипотентные мезенхимальные стромальные клетки кролика модифицировали при помощи среды для хондрогенной дифференцировки StemPro или рекомбинантного белка TGF-β3. Экспрессию генов, ответственных за хондрогенез (Acan, Tgfβ3, Col2α1, Comp), измеряли с помощью полимеразной цепной реакции в реальном времени (real-time PCR) методом ΔΔCt.

Результаты. Показано, что дермальные фибробласты человека способны к хондрогенной модификации с помощью белковых сред. Данный тип клеток легко доступен для забора, не требует особых условий при культивировании, легко масштабируется, а также может быть использован для аллогенной трансплантации.

Заключение. Полученные данные можно использовать при конструировании тканеинженерных продуктов для регенерации гиалинового хряща на основе аллогенных дермальных фибробластов.

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