Genes & CellsGenes & Cells2313-18292500-2562Human Stem Cells Institute27917110.23868/gc279171Research ArticlePerculiarities of physiologic and reparative osteogenesis following the transfusion of bone marrow mononuclear cellsDeevR. V.redaktor@celltranspl.ruTsupkinaN. V.redaktor@celltranspl.ruSergeevV. S.redaktor@celltranspl.ruSerikovV. B.redaktor@celltranspl.ruGololobovV. G.redaktor@celltranspl.ruPinaevG. P.redaktor@celltranspl.ruKirov Military Medical AcademyRAS Institution of Cytology (Cellular Cultures Department)Children’s Hospital Oakland Research Institute150320061354582402202324022023Copyright © 2006, Eco-Vector2006<p>Physiologic and reparative regeneration of bone tissue after transfusion of bone marrow GFP-positive mononuclear cells of mice C57B1 /6-TgN(ACTbGFP)1 Osb was studied in C57B1/6 mice exposed to radiation. It is shown that transplanted cells are able to differentiate into osteoblasts and osteocytes in both physiologic and reparative osteogenesis, these cells having priority to proliferation and differentiation in the recipient exposed to radiation. The GFP-positive osteocytes number within the substance reached 318%.</p>bone tissueosteogenesisregenerationgreen fluorescenting proteinкостная тканьостеогенезрегенерациязеленый флюоресцирующий белок[Wang X., Li F., Niyibizi C. Progenitors systemically transplanted into neonatal mice localize to areas of active bone formation in vivo: implications of cell therapy for skeletal diseases. Stem Cells 2006; 24(8): 1869-78.][Horwitz E.M., Prockop D.J., Gordon P.L. et al. Clinical responses to bone marrow transplantation in children with severe osteogenesis imperfecta. Blood 2001; 97(5): 1227-31.][Horwitz E.M., Gordon P.L., Koo W.K. et al. Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone. Proc. Natl. Acad. Sci. USA 2002; 99(13): 8932-7.][Owen M.E., Friedenstein A.J. Stromal stem cells : marrow-derived osteogenic precursors. Cell and molecular biology of vertebrate hard tissues. Proceedings of a symposium held at the Ciba Foundation. London. Oct. 13-15, 1987. London: John Wiley & Sons. 1988: 42-53.][Horwitz E., Le Blanc K., Dominici M. et al. Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy 2005; 7(5): 393-5.][Деев P.B., Цупкина H.B., Сериков В.Б., Гололобов В.Г., Пинаев ГЛ. Участие трансфузированных клеток костного мозга в репаративном остеогистогенезе. Цитология 2005; 46(9): 755-9.][Jiang X., Kalajzic Z., Maye P. et al. Histological Analysis of GFP Expression in Murine Bone. J. Histochem. Cytochem. 2005; 53(5): 593-602.][Bilic-Curcic I., Kronenberg M., Bellizzi J. et al. Visualizing levels of osteoblast differentiation by a two color promoter-GFP strategy: type I collagen-GFPcyan and osteocalcin GFPtpz. Genesis 2005; 43: 87-98.][Wang L., Liu Y., Kalajzic Z. et al. Heterogeneity of engrafted bone-lining cells after systemic and local transplantation. Blood 2005; 106(10): 3650-7.][Dominicib M., Pritchardb C., Garlits J.E. et al. Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation. Proc. Natl. Acad. Sci. USA 2004; 101 (32): 11761 -6.][Гололобов В.Г., Деев P.В. Стволовые стромальные клетки и остеобластический клеточный дифферон. Морфология 2003; 123(1 ): 9-19.][Schirrmacher K., Smitz I., Winterhager E. et al. Caracterization of gap junctions between osteoblast-like cells in culture. Calif. Tiss. Int. 1992; 51: 285-90.][Frost H.M. Treatment of osteoporoses by manipulation of coherent bone cell populations. Clin. Orthop. 1979; 143: 227-44.][Dempster D.W. Ремоделирование кости. Остеопороз. М., СПб.: Бином, Невский диалект, 2000: 85-108.][Гололобов В.Г., Дулаев А.К., Деев Р.В., Цыган E.H. Морфофункциональная организация, реактивность и регенерация костной ткани / Под ред. проф. Р.К. Данилова, проф. В.М. Шаповалова. СПб.: ВМедА, 2006: 47.][Ponder K.P. Gene therapy for hemophilia. Curr. Opin. Hematol. 2006; 13(5): 301-7.][Hasegawa N., Kawaguchi H., Hirachi A. et al. Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects. J. Periodontol. 2006; 77(6): 1003-7.][Piersanti S., Sacchetti B., Funari A. et al. Lentiviral transduction of human postnatal skeletal (stromal, mesenchymal) stem cells: in vivo transplantation and gene silencing. Calcif. Tissue Int. 2006; 78(6): 372-84.]