Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute23803310.23868/gc238033Original Study ArticlesОригинальные исследованияResearch ArticleThe effect of severe polytrauma on the migration of hematopoietic stem cells in miceВлияние тяжелой политравмы на миграцию стволовых кроветворных клеток у мышейAlexandrovV. N.АлександровВ. Н.
Russian Federation
redaktor@celltranspl.ruSergeevV. S.СергеевВ. С.
Russian Federation
redaktor@celltranspl.ruS.M. Kirov Military Medical AcademyВоенно-медицинская академия им. С.М. Кирова150220061259621502202315022023Copyright ©; 2006, Eco-VectorCopyright ©; 2006, Эко-Вектор2006Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/238033

It has been shown that hematopoietic stem cells (SCCS) are involved in the physiological and reparative regeneration of non-hematopoietic tissues and organs through transdifferentiation and/or cell fusion. In this regard, it seems interesting to evaluate the kinetics of bone marrow CCM in severe trauma. Three experimental models were used in the study: injury to mice followed by uneven irradiation in lethal doses with 1/2 shin screening (A), irradiation of mice in lethal doses followed by transplantation of bone marrow cells from injured mice (B), injury to mice followed by irradiation in sublethal doses (C). The migration of CCM was assessed by registering hematopoietic colonies growing on the spleen of irradiated mice from endo- or exogenous SCCS. In experimental models A and B, it was shown that a significantly larger number of endocolonies are formed in the spleens of injured mice in comparison with mice of control groups. In the experimental model B, it was shown that the bone marrow of injured mice contains a significantly smaller number of CCM capable of migration. Based on the results obtained, it was concluded that severe polytrauma induces CCM migration in mice.

Показано, что стволовые кроветворные клетки (СКК] участвуют в физиологической и репаративной регенерации негематопоэтических тканей и органов посредством трансдифференцировки и/или клеточного слияния. В связи с этим представляется интересным оценить кинетику СКК костного мозга при тяжелой травме. В исследовании использовали три экспериментальные модели: травмирование мышей с последующим неравномерным облучением в летальных дозах с экранированием 1/2 голени (А), облучение мышей в летальных дозах с последующей трансплантацией клеток костного мозга от травмированных мышей (Б), травмирование мышей с последующим облучением в сублетальных дозах (В). Миграцию СКК оценивали путем регистрации кроветворных колоний, вырастающих на селезенке облученных мышей из эндо- или экзогенных СКК. В экспериментальных моделях А и В показано, что в селезенках травмированных мышей формируется достоверно большее количество эндоколоний в сравнении с мышами групп контроля. В экспериментальной модели Б показано, что костный мозг травмированных мышей содержит достоверно меньшее количество способных к миграции СКК. На основании полученных результатов сделан вывод, что тяжелая политравма индуцирует миграцию СКК у мышей.

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