Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute22997710.23868/gc229977Original Study ArticlesОригинальные исследованияResearch ArticleAre oval cells supposed to be liver stem cells or hepatoblasts?Овальные клетки - предполагаемые стволовые клетки печени или гепатобласты?KiyasovА. P.КиясовА. П.
Russian Federation

Department of Normal Anatomy

кафедра нормальной анатомии

redaktor@celltranspl.ruGumerovaA. A.ГумероваА. А.
Russian Federation

Department of Normal Anatomy

кафедра нормальной анатомии

redaktor@celltranspl.ruTitovaM. A.ТитоваМ. А.
Russian Federation

Department of Normal Anatomy

кафедра нормальной анатомии

redaktor@celltranspl.ruKazan State Medical UniversityКазанский государственный медицинский университет150220061255581302202313022023Copyright ©; 2006, Eco-VectorCopyright ©; 2006, Эко-Вектор2006Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/229977

The aim of the work is to study the patterns of expression of oval cell markers during prenatal histogenesis of rat and human liver, as well as in human hepatocytes in vitro. The liver of rats was obtained at various gestation periods and during the first month after birth. Human embryos and fetuses are obtained as a result of legal medical abortions. The material was fixed and poured into paraffin according to the standard procedure or cryostatic sections were prepared, then stained with immunohistochemical indirect immunoperoxidase and streptavidin-biotin methods using commercial monoclonal antibodies to cytokeratins-7, -8, -18, -19. Gamma-glutamyl-transpeptidase was detected histochemically. The results of the studies showed that rat and human hepatoblasts express cytokeratin-19 and gamma-glutamyltrans-peptidase, whereas cholangiocytes express cytokeratin-7 (and only since the beginning of the formation of intrahepatic bile ducts). Moreover, human hepatocytes under cultivation begin to express cytokeratin-19 again. The data obtained show that the markers of oval cells (cytokeratin-19 and gamma-glutamyltranspeptidase) are markers of differentiating hepatocytes, which does not allow identifying oval cells with liver stem cells.

Цель работы - изучение закономерностей экспрессии маркеров овальных клеток в ходе пренатального гистогенеза печени крысы и человека, а также в гепатоцитах человека in vitro. Печень крыс получали на различных сроках гестации и в течение первого месяца после рождения. Эмбрионы и плоды человека получены в результате легальных медицинских абортов. Материал фиксировали и заливали в парафин по стандартной методике или готовили криостатные срезы, далее окрашивали иммуногистохимическим непрямым иммунопероксидазным и стрептавидин-биотиновым методами с использованием коммерческих моноклональных антител к цитокератинам-7, -8, -18, -19. Гамма-глутамил- транспептидазу выявляли гистохимически. Результаты исследований показали, что гепатобласты крысы и человека экспрессируют цитокератин-19 и гамма-глутамилтранс- пептидазу, тогда какхолангиоциты экспрессируют цитокератин-7 (и только с момента начала образования внутрипеченочныхжелчных протоков). Более того, гепатоциты человека в условиях культивирования вновь начинают экспрессировать цитокератин-19. Полученные данные показывают, что маркеры овальных клеток (цитокератин-19 и гамма-глутамилтранспептидаза) являются маркерами дифференцирующихся гепатоцитов, что не позволяет отождествлять овальные клетки со стволовыми клетками печени.

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