Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12198710.23868/202012010ArticlesСтатьиResearch ArticleResults of a comparative valuation of the efficiency of using the plasmid construct pBud-VEGF165-FGF2 in models of autograft of the sciatic nerve defect and tubulation with the NeuraGen® collagen tubeРезультаты сравнительной оценки эффективности применения плазмидной конструкции pBud-VEGF165-FGF2 в моделях аутонервной пластики дефекта седалищного нерва и тубуляции коллагеновой трубкой NeuraGen®MasgutovR. FМасгутовР. ФMasgutovaG. AМасгутоваГ. АMukhametovaL. RМухаметоваЛ. РIdrisovaK. FИдрисоваК. ФMullakhmetovaA. FМуллахметоваА. ФSyromiatnikovaV. YСыромятниковаВ. ЮBogovA. AБоговА. АSalafutdinovI. IСалафутдиновИ. ИArkhipovaS. SАрхиповаС. СSalikhovR. ZСалиховР. ЗRizvanovA. AРизвановА. АKazan (Volga region) Federal UniversityКазанский (Приволжский) федеральный университетRepublican Clinical Hospital, KazanРеспубликанская клиническая больница, Казань10122020154VOL 15, NO4 (2020)ТОМ 15, №4 (2020)616516012023Copyright ©; 2020, Eco-VectorCopyright ©; 2020, Эко-Вектор2020Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121987

Traumatic injuries of peripheral nerves lead to profound disability in patients with partial or total loss of limb function. There remains the question about the use of technologies for detecting defects of the peripheral nerve with concurrent of its regeneration. In the study it has been investigated the effect of the gene-therapeutic plasmid construct pBud-VEGF165-FGF2 with various methods of overcoming 5 mm diastasis of the sciatic nerve: nerve autograft and tubulation with the NeuraGen® tube. In the study groups, assessment of sciatic nerve regeneration was based on functional and morphometric parameters. Direct injection of plasmid pBud-VEGF165-FGF2 stimulates regeneration and restoration of motor function in both groups, but with different efficacy. Comparative analysis of nerve defect replacement in combination with direct gene therapy showed the most effective approach with autologous insertion replacement by comparison to the NeuraGen. Thus, on the basis of the obtained data, we can assert that nerve autograft of the peripheral nerve remains the "gold standard” and provides the best hope of research in combination with the use of various regeneration stimulants.

Травматические повреждения периферических нервов зачастую приводят к глубокой инвалидизации пациентов с частичной или полной утратой функции конечностей. Остается открытым вопрос о применении технологий замещения дефектов периферического нерва с одновременной стимуляцией его регенерации. Цель работы: оценка эффективности применения геннотерапевтической плазмидной конструкции рBud-VEGF165-FGF2 при замещении 5 мм диастаза седалищного нерва крысы методом аутонервной вставки или тубуляции трубкой NeuraGen®. Регенерацию седалищного нерва оценивали по функциональным и морфометрическим параметрам. Прямое введение плазмиды рBud-VEGF165-FGF2 стимулировало регенерацию седалищного нерва и восстановление двигательной функции в исследуемых группах с разной эффективностью. Анализ результатов посттравматического восстановления выявил, что замещение дефекта cедалищного нерва путем аутологичной вставки в сочетании с прямой генной терапией наиболее эффективно. Таким образом, аутонервная пластика периферического нерва остается «золотым стандартом» и является наиболее перспективной моделью исследования в комбинации с различными стимуляторами регенерации.

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