Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12197110.23868/202012005ArticlesСтатьиReview ArticleNon-viral delivery of the BMP2 gene for bone regenerationНевирусная доставка гена BMP2 для регенерации костной тканиNedorubovaI. AНедорубоваИ. Аnedorubova.ia@gmail.comBukharovaT. BБухароваТ. БVasilyevA. VВасильевА. ВGoldshteinD. VГольдштейнД. ВKulakovA. AКулаковА. АResearch Centre for Medical GeneticsМедико-генетический научный центр им. академика Н.П. БочковаCentral Research Institute of Dental and Maxillofacial SurgeryНациональный медицинский исследовательский центр «Центральный научно-исследовательский институт стоматологии и челюстно-лицевой хирургии»10122020154VOL 15, NO4 (2020)ТОМ 15, №4 (2020)333916012023Copyright ©; 2020, Eco-VectorCopyright ©; 2020, Эко-Вектор2020Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121971

Gene-activated bone grafts and substitutes are promising tools for the bone defect healing, which are capable to induce prolonged production of growth factors with a therapeutic effect at physiological concentrations. Non-viral methods of delivering plasmid constructs with target genes are the safest for clinical use, but their efficiency is lower in comparison with viral vectors. To solve the problem of plasmid delivery into cells, some systems with a high transfection capacity and ensure sufficient cell viability are being developed. Moreover, there are different approaches to improve the level of expression of target genes and targeted delivery to the bone defect in order to achieve local therapeutic concentrations. This review considers approaches which are aimed to increase the efficiency of bone tissue regeneration methods based on non-viral delivery systems for osteoinduction genes using the example of the bone morphogenetic protein-2 gene.

Ген-активированные костно-пластические материалы являются перспективным инструментом для заживления костных дефектов, они способны индуцировать пролонгированную продукцию факторов роста с терапевтическим действием в физиологических концентрациях. Наиболее безопасны для клинического применения невирусные способы доставки плазмидных конструкций с целевыми генами, но их эффективность ниже по сравнению с вирусными векторами. Для решения проблемы доставки плазмид в клетки ведется разработка систем с высокой трансфицирующей активностью, обеспечивающих достаточную жизнеспособность клеток. Кроме того, используются подходы для повышения уровня экспрессии целевых генов и их адресной доставки в зону костного дефекта с целью достижения локальных терапевтических концентраций. В настоящем обзоре рассмотрены подходы, направленные на повышение эффективности методов регенерации костной ткани, основанных на невирусных системах доставки генов индукторов остеогенеза на примере гена костного морфогенетического белка-2 - BMP-2.

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