Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12177210.23868/gc121772ArticlesСтатьиNeural markers in investigation of stem cells differentiationНейральные маркеры, используемые при изучении дифференцировки стволовых клетокKorzhevskiyD EКоржевскийД Э

НИИ Экспериментальной медицины СЗО РАМН, Санкт-Петербург

PetrovaE SПетроваЕ С

НИИ Экспериментальной медицины СЗО РАМН, Санкт-Петербург

KirikOBКирикОБ

НИИ Экспериментальной медицины СЗО РАМН, Санкт-Петербург

BezninG VБезнинГ В

НИИ Экспериментальной медицины СЗО РАМН, Санкт-Петербург

SukhorukovaE GСухоруковаЕ Г

НИИ Экспериментальной медицины СЗО РАМН, Санкт-Петербург

KorzhevskiiO E

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

PetrovaE S

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

KirikO V

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

BezninG V

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

SuchorukovaE G

Institute for experimental medicine of Russian academy of medical sciences, Saint Petersburg

НИИ Экспериментальной медицины СЗО РАМН, Санкт-ПетербургInstitute for experimental medicine of Russian academy of medical sciences, Saint Petersburg1509201053NO3 (2010)№3 (2010)576311012023Copyright ©; 2010, Eco-VectorCopyright ©; 2010, Эко-Вектор2010Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121772

Investigation of stem cells differentiation substantiates the reasonability of use and confirms clinical effectiveness of new developments in cellular technology. Immunocytochemical methods of revealing of synthesized marker proteins are widely used in evaluation of stem cells differentiation. This review contains characteristics of the most often used markers of neural differentiation that are used in investigation of histoblastic potencies of stem [including neural stem/progenitor) cells. These neuromarkers include nuclear protein of nerve cells NeuN, beta-tubulin III, protein MAP2 associated with microtubules, proteins of neurofilaments, neuron-specific enolase, synaptophysin and molecule of nerve cells adhesion CPSA-NCAM] connected to polysialic acid. The study evaluates the specificity of these markers and reveals the advantages and disadvantages of methods used for their detection. It is emphasized that detection of some proteins expression cannot serve as an unequivocal evidence of stem cells differentiation into neurons. The most neuronspecific markers are: NeuN, neurofilament proteins and synaptophysin.

Изучение дифференцировки стволовых клеток позволяет обосновать целесообразность использования и подтвердить клиническую эффективность новых разработок в области клеточных технологий. В настоящее время для оценки дифференцировки стволовых клеток широко используются методы иммуноцитохимического выявления синтезируемых ими маркерных белков. В настоящем обзоре дана характеристика наиболее часто применяемых маркеров нейральной дифференцировки, используемых при исследовании гистобласти-ческих потенций стволовых Св том числе нейральных стволо-вых/прогениторных] клеток. К таким нейромаркерам относятся ядерный белок нервных клеток NeuN, [i-тубулин III, ассоциированный с микротрубочками белок МАР2, белки нейрофи-паментов, нейрон-специфическая енолаза, синаптофизин и связанная с полисиаловой кислотой молекула адгезии нервных клеток CPSA-NCAM]. В работе проведена оценка специфичности этих маркеров, выявлены преимущества и недостатки использования методов их выявления. Подчеркивается, что обнаружение экспрессии некоторых белков не может служить однозначным свидетельством дифференцировки стволовых клеток именно в нейроны. Наиболее нейронспе-цифичными являются такие маркеры, как NeuN, белки ней-рофиламентов и синаптофизин.

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