Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12172110.23868/gc121721ArticlesСтатьиLife without water: cryptobiosis of invertebrates as a model for next generation techmologyof biomaterials preservationЖизнь без воды: криптобиоз беспозвоночныхкак модель для разработки технологии консервациибиоматериала нового поколенияShagimardanova,E IШагимардановаЕ И

Kazan (Volga region) federal university, Kazan

Казанский (Приволжский) федеральный университет, Казань

Sharipova,M RШариповаМ Р

Kazan (Volga region) federal university, Kazan

Казанский (Приволжский) федеральный университет, Казань

Rizvanov,A AРизвановА А

Kazan (Volga region) federal university, Kazan

Казанский (Приволжский) федеральный университет, Казань

Zaharov,I SЗахаровИ С

Kazan (Volga region) federal university, Kazan

Казанский (Приволжский) федеральный университет, Казань

GusevO AГусевО А

Kazan (Volga region) federal university, Kazan

Казанский (Приволжский) федеральный университет, Казань

Kazan (Volga region) federal university, KazanКазанский (Приволжский) федеральный университет, Казань1509201273NO3 (2012)№3 (2012)18518911012023Copyright ©; 2012, Eco-VectorCopyright ©; 2012, Эко-Вектор2012Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121721

To date, advances in the field of tissue engineering, cell transplantation and genetic engineering have made the biological materials of different origin an important therapeutic tool in clinical medicine. Currently, cells preservation is achieved by freezing at -80°С or in liquid nitrogen. Cryopreservation technology is expensive and has considerable limits during transportation. Preservation of viable biological material in dry state under ambient temperature is considered as attractive, but yet fully achieved alternative. There are organisms which are able to survive complete water loss. Understanding of mechanisms underlying dehydration tolerance will allow the development of dry preservation technology for molecules, cells and organs, and further use of these methods in medicine, pharmacology and biotechnology.

Достижения в области клеточной трансплантологии, генной и тканевой инженерии делают биологический материал различного происхождения важным терапевтическим инструментом в клинической практике. Сохранение жизнеспособности клеток в настоящее время достигается их замораживаем при -80°С или в жидком азоте. Технология криозаморозки является дорогостоящей и имеет значительные ограничения при транспортировке биоматериала. Альтернативой криотехнологии может служить сохранение биологического материала в обезвоженном состоянии при комнатной температуре. Существует ряд организмов, способных выживать при полной потере воды. Знание механизмов, лежащих в основе толерантности к обезвоживанию, позволит разработать технологии хранения молекул, клеток и органов млекопитающих в обезвоженном состоянии для их дальнейшего использования в медицине, фармакологии и биотехнологии

cryptobiosiscell culturedehydrationкриптобиозкультуры клетокобезвоживаниеJulca I., Alaminos M., González-López J. et al. Xeroprotectants for the stabilization of biomaterials. Biotechnol. Adv. 2012; in press.Wakayama T., Yanagimachi R. Development of normal mice from oocytes injected with freeze-dried spermatozoa. Nat. Biotechnol. 1998; 16(7): 639-41.Loi P., Matzukawa K., Ptak G. et al. Nuclear transfer of freezedried somatic cells into enucleated sheep oocytes. Reprod. Domest. Anim. 2008; 43: 417-22.Quan G.B., Han Y., Liu M.X. et al. Addition of oligosaccharide decreases the freezing lesions on human red blood cell membrane in the presence of dextran and glucose. Cryobiology 2011; 62(2): 135-44.Zhou X.L., Zhu H., Zhang S.Z. et al. Freeze-drying of human platelets: influence of intracellular trehalose and extracellular protectants. Cryo. Letters. 2006; 27: 43-50.Ono T., Mizutani E., Li C. et al. Nuclear transfer preserves the nuclear genome of freeze-dried mouse cells. J. Reprod. Dev. 2008; 54(6): 486-91.García de Castro A., Tunnacliffe A. Intracellular trehalose improves osmotolerance but not desiccation tolerance in mammalian cells. FEBS Lett. 2000; 487(2): 199-202.Eroglu A., Russo M.J., Bieganski R et al. Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nat. Biotechnol. 2000; 18(2): 163-7.Lynch A.L., Slater N.K. Influence of intracellular trehalose concentration and pre-freeze cell volume on the cryosurvival of rapidly frozen human erythrocytes. Cryobiology 2011; 63(1): 26-31.Buchanan S.S., Pyatt D.W., Carpenter J.F. Preservation of differentiation and clonogenic potential of human hematopoietic stem and progenitor cells during lyophilization and ambient storage. PLoS One 2010; 5(9): 12518.Guo N., Puhlev I., Brown D.R. et al. Trehalose expression confers desiccation tolerance on human cells. Nat. Biotechnol. 2000; 18(2): 168-71.Chakraborty N., Menze M.A., Elmoazzen H. et al. Trehalose transporter from African chironomid larvae improves desiccation tolerance of Chinese hamster ovary cells. Cryobiology 2012; 64(2): 91-6Elliott G.D., Liu X.H., Cusick J.L. et al. Trehalose uptake through P2X7 purinergic channels provides dehydration protection. Cryobiology 2006; 52(1): 114-27.Eroglu A., Lawitts J.A., Toner M. Quantitative microinjection of trehalose into mouse oocytes and zygotes, and its effect on development. Cryobiology 2003; 46(2): 121-34.Hubel A., Darr T.B. Passive loading of tregalose into cells. Cryobiology 2000; 45: 227.Chakraborty N., Biswas D., Parker W. et al. A role for microwave processing in the dry preservation of mammalian cells. Biotechnol. Bioeng. 2008; 100(4): 782-96.Clegg J.S. Cryptobiosis - a peculiar state of biological organization. Comp. Biochem. Physiol. B. 2001; 128: 613-24.Rebecchini L., Altiero T., Guidetti R. Anhydrobiosis: the extreme limit of dessication tolerance. Invert. Surv. J. 2007; 4: 65-81.Nelson D.R. Current status of the tardigrada: evolution and ecology. Integr. Comp. Biol. 2002; 42: 652-59.Watanabe M. Anhydrobisis in invertebrated. Appl. Entom. Zool. 2006; 41: 15-31.Watanabe M., Kikawada T., Minagawa N. et al. Mechanism allowing an insect to survive complete dehydration and extreme tempetatures. 2002. J. Exp. Biol. 205: 2799-802.Cornette R., Kikawada T. The induction of anhydrobiosis in the sleeping chironomid: current status of our knowledge. IUBMB Life 2011; 63(6): 419-29.Watanabe M., Kikawada T., Okuda T. Increase of internal ion concentration triggers trehalose synthesis associated with cryptobiosis in larvae of Polypedilum vanderplanki. J. Exp. Biol. 2003; 206: 2281-6.Carpenter J.F., Crowe J.H. An infrared spectroscopic study of the interactions of carbohydrates with dried proteins. Biochemistry 1989; 28(9): 3916-22.Oku K., Watanabe H., Kubota M. et al. NMR and quantum chemical study on the OH...pi and CH...O interactions between trehalose and unsaturated fatty acids: implication for the mechanism of antioxidant function of trehalose. J. Am. Chem. Soc. 2003; 125(42): 12739-48.Berjak P., Farrant J.M., Pammenter N.W. Seed desiccationtolerance mechanism. In: Jenks M.A., and Wood A.J., editors. Plant Desiccation Tolerance. Blackwell, Oxford; 2007. p 151-92.Gusev O., Cornette R., Kikawada T. et al. Expression of heat shock protein-coding genes associated with anhydrobiosis in an African chironomid Polypedilum vanderplanki. Cell Stress & Chaperones 2011; 16(1): 81-90.Schill R.O., Steinbruck G.H., Kohler H.R. Stress gene (hsp70) and quantitative expression in Milnesium tardigradum (Tardigrada) during active and cryptobitic stages. J. Exp. Biol. 2004; 207:1607-13.Liang P., MacRae T.H. The synthesis of a small heat shock/ alpha-crystallin protein in Artemia and its relationship to stress tolerance during development. Dev. Biol. 1999; 207: 445-56.Reuner A., Hengherr S., Brahim M. et al. Stress responce in tardigrades: differential gene expression of molecular shaperones. Cell Stress Chaperones 2010; 15: 423-30.Hong-Bo S., Zong-Suo L., Ming-An S. LEA proteins in higher plants: structure, function, gene expression and regulation. Colloids Surf B Biointerfaces 2005; 45(3-4): 131-5.Browne J., Tunnacliffe A., Burnell A. Plant desiccation gene found in a nematode. Nature 2002; 416: 38.Hand S.C., Menze M.A., Toner M. et al. LEA proteins during water stress: not just for plants anymore. Annu. Rev. Physiol. 2011; 73: 115-34.Tunnacliffe A., Wise M. The continuing conundrum of the LEA proteins. Naturwissenschaften 2007; 94: 791-812.Goyal K., Walton L.J., Tunnacliffe A. LEA proteins prevent protein aggregation due to water stress. Biochem. J. 2005; 338: 151-7.Cornette R., Kanamori Y., Watanabe M. et al. Identification of anhydrobiosis-related genes from an expressed sequence tag database in the cryptobiotic midge Polypedilum vanderplanki (diptera; chironomidae). J. Biol. Chem. 2010: 285: 35889-99.Schokraie E., Hotz-Wagenblatt A., Warnken U. et al. Proteomic analysis of tardigrades: towards a better understanding of molecular mechanisms by anhydrobiotic organisms. PlosOne 2010; 5:e9502.Gusev O., Nakahara Y., Vanyagina V. et al. Anhydrobiosisassociated nuclear DNA damage and repair in the sleeping chironomid: Linkage with radioresistance. PLoS ONE 2010; 5(11): e14008.Jenks M.A., Wood A.J. Plant desiccation tolerance. Ames, Iowa: Blackwell Pub; 2007.Reardon W., Chakrabortee S., Pereira T.G. et al. Expression profiling and cross-species RNA interference (RNAi) of desiccationinduced transcripts in the anhydrobiotic nematode Aphelenchus avenaue. BMC Mol. Biol. 2010; 11: 6.Gladyshev E., Meselson M. Extreme resistance of bdelloid rotifers to ionizing radiation. PNAS USA 2008; 105: 5139-44.Newmann S., Reuner A., Brummer F. et al. DNA damage in storage cells of anhydrobiotic tardigrades. Comp. Biochen. Physiol. Mol. Integr. Physiol. 2009; 153: 425-29.Cashio P., Lee T.V., Bergmann A. Genetic control of programmed cell death in Drosophilla melanogaster. Semin. Cell Dev. Biol. 2005; 16: 225-35.Natan D., Nagler A., Arav A. Freeze-drying of mononuclear cells derived from umbilical cord blood followed by colony formation. PLoS One 2009; 4(4): e5240.Sasnoor L.M., Kale V.P., Limaye L.S. Prevention of apoptosis as a possible mechanism behind improved cryoprotection of hematopoietic cells by catalase and trehalose. Transplantation 2005; 80(9): 1251- 60.Sasnoor L.M., Kale V.P., Limaye L.S. A combination of catalase and trehalose as additives to conventional freezing medium results in improved cryoprotection of human hematopoietic cells with reference to in vitro migration and adhesion properties. Transfusion 2005; 45(4): 622-33.