Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12160410.23868/gc121604ArticlesСтатьиResearch ArticleComparison of different methods for generation of functional human cardiomyocytesСравнение эффективности методов получения функционально активных кардиомиоцитов человекаKhudiakovA. AХудяковА. А.KurapeevD. IИ КурапеевД. IKostarevaA. AА КостареваА. AMalashichevaA. BБ МалашичеваА. BFederal Almazov heart blood and endocrinology centre, Saint-PetersburgФедеральный Центр сердца, крови и эндокринологии им. В.А. Алмазова МЗ РФ, Санкт-Петербург1508201382VOL 8, NO2 (2013)ТОМ 8, №2 (2013)475511012023Copyright ©; 2013, Eco-VectorCopyright ©; 2013, Эко-Вектор2013Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121604

Generation of human cardiomyocytes in vitro is important for basic science and for regenerative medicine. There are a lot of published protocols describing the cardiomyocytes obtaining with different efficacy. However in practice, a researcher is faced with the fact that published methods have to be adapted to a particular laboratory for specific tasks. The aim of this study was to compare three methods for generation of human cardiomyocytes in vitro: 1) from human adult heart sample by purifying myocardial progenitor cells and their subsequent differentiation into cardiomyocyte direction; 2) from human adipose tissue multipotent mesenchimal stromal cells (AT-MSC); 3) from human dermal fibroblasts using reprogramming technology and subsequent differentiation to cardiomyocytes. We have shown relatively low efficiency of cardiac progenitor cells and AT-MSC capacity to give rise to cardiomyocyte lineage. The effectiveness of cardiomyocytes differentiation from dermal fibroblasts by reprogramming technology was significantly higher. The advantages, disadvantages and perspectives of each method are discussed.

Задача получения кардиомиоцитов человека in vitro важна как с точки зрения фундаментальной науки, так и для «регенеративной медицины». Опубликовано множество протоколов получения культуры кардиомиоцитов той или иной степени эффективности, однако на практике каждый исследователь сталкивается с тем, что опубликованные методики приходится адаптировать в конкретной лаборатории для конкретных задач. Целью настоящей работы было сравнить три метода получения кардиомиоцитов в культуре: 1) из образца миокарда путём получения клеток-предшественниц и их последующей дифференцировки в кардиомиоцитарном направлении; 2) из мультипотентных мезенхимных стромальных клеток жировой ткани; 3) из фибробластов кожи человека путём их репрограммирования и последующей дифференци-ровки в кардиомиоциты c использованием опубликованного ранее протокола [18] c авторскими модификациями. Показана невысокая эффективность получения кар-диомиоцитов в культуре из клеток-предшественниц миокарда взрослого человека и из мультипотентных мезенхимных стромальных клеток; эффективность получения кардиомиоцитов с применением репрограммированных клеток оказалась значительно выше. Обсуждены преимущества и недостатки каждого метода и перспективы их использования.

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