Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12160110.23868/gc121601ArticlesСтатьиResearch ArticleA comparative study of the influence of skin fibroblasts and bone marrow stromal cells included in collagen gel on gingiva regenerationСравнительное исследование влияния дермальных фибробластов и мультипотентных мезенхимных стромальных клеток, заключенных в коллагеновый гель, на регенерацию десныBarmashevaA. AБармашеваА. АNikolaenkoN. SНиколаенкоН. СSamusenkoI. AСамусенкоИ. АOrekhovaL. YuОреховаЛ. ЮPinaevG. PПинаевГ. ПP. Pavlov Saint-Petersburg State Medical University, Saint-PetersburgСанкт-Петербургский Государственный медицинский университет им. акад. И.П. Павлова, Санкт-ПетербургInstitute of Cytology, Saint-PetersburgИнститут Цитологии РАН, Санкт-ПетербургA.M. Nikiforov Russian Center of Urgent and Radiation Medicine, Saint-PetersburgВсероссийский центр экстренной и радиационной медицины им. А.М. Никифорова МЧС России, Санкт-Петербург1508201382VOL 8, NO2 (2013)ТОМ 8, №2 (2013)354311012023Copyright ©; 2013, Eco-VectorCopyright ©; 2013, Эко-Вектор2013Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121601

The aim of this research was to compare the influence of dermal fibroblasts and multipotent mesenchymal stromal cells included in type I collagen gel on oral mucosa regeneration. It was shown that cultured cells included in collagen gel allowed receiving the greater volume of soft tissues in 180 days than a standard technique of recession management. Repaired gingiva had a normal histological morphology.

Целью настоящей работы являлось сравнение эффективности применения дермальных фибробластов и мультипотентных мезенхимных стромальных клеток, заключенных в коллагеновый гель, при хирургическом вмешательстве по устранению дефицита прикрепленной слизистой полости рта на модели рецессии десны. Показано, что применение культивированных клеток, заключенных в коллагеновый гель, позволяет получить к 180 сут. наблюдения больший объем десны, чем применение стандартной методики устранения рецессии. Гистологическое строение восстановленной десны соответствовало норме.

dermal fibroblastsmultipotent mesenchymal stromal cellscollagengingivarecessionдермальные фибробластымульти-потентные мезенхимные стромальные клеткиколлагенрецессиядеснаKassab M.M., Cohen R.E. The etiology and prevalence of gingival recession. J Am. Dent. Assoc. 2003; 134t2):220—5.Albandar J.M., Kingman A. Gingival recession, gingival bleeding, and dental calculus in adults 30 years of age and older in the United States, 1988—1994. J Periodontol. 1999;70t1): 30—43.Serino G., Wennstrom J.L., Lindhe J. et al. The prevalence and distribution of gingival recession in subjects with a high standard of oral hygiene. J. Clin. Periodontol. 1994; 21(1): 57—63.Roman A., Louise F., M'barek R. et al. Gingival recessions: epidemiologic, etiologic and therapeutic aspects. Inter. J. Dent. Sci. 2009;7 (1).Susin C., Haas A.N., Oppermann R.V. et al. Gingival recession: epidemiology and risk indicators in a representative urban Brazilian population. J. Periodontol. 2004; 75(10): 1377—86.Hosanguan C., Ungchusak C., Leelasithorn S. et al. The extent and correlates of gingival recession in non-institutionalised Thai elderly. J. Int. Acad. Periodontol. 2002; 4(4): 143—8Kozlowska M., Wawrzyn-Sobczak K., Karczewski J.K. et al. The oral cavity hygiene as the basic element of the gingival recession prophylaxis. Rocz. Akad. Med. Bialymst. 2005; 50(Suppl 1): 234—7.Marini M.G., Greghi S.L., Passanezi E. et al. Gingival recession: prevalence, extension and severity in adults. J. Appl. Oral Sci. 2004; 12(3): 250-5.Miller P.D. A classification of marginal tissue recession. Int. J. Periodontics Restor. Dent. 1985; 5: 8-13.Rosetti E.P., Marcantonio R.A., Rossa C. et al. Treatment of gingival recession: comparative study between subepithelial connective tissue graft and guided tissue regeneration. J. Periodontol. 2000; 71: 1441-7.Toscano N., Holtzclaw D., Victor S. A prospective pilot study on the clinical application of stromal vascular fraction stem cells in the treatment of miller class 1 and 2 recession gingival defects. J. Implant. Adv. Clin. Dent. 2011; 3(6): 23-33.Cortellini P., Clauser C., Pini-Prato G.P. Histologic assessment of new attachment following the treatment of a human buccal recession by means of a guided tissue regeneration procedure. J. Periodontol. 1993;64: 387-91.Parma-Benfenati S., Tinti C. Histologic evaluation of new attachment utilizing a titanium-reinforced barrier membrane in a mucogingival recession defect. A case report. J. Periodontol. 1998; 69: 834-9.Trombelli L., Schincaglia G.P., Scapoli C. et al. Healing response of buccal gingival recessions treated with expanded polytetrafluoroethylene membranes. A retrospective report. J. Periodontol. 1995; 66: 14-22.Nickels K., Ratka-Kruger P., Neukranz E. et al. Ten-year results after connective tissue grafts and guided tissue regeneration for root coverage. J. Periodontol. 2010; 81: 827-36.Pini Prato G.P., Tinti C., Vincenzi G. et el. Guided tissue regeneration versus mucogingival surgery in the treatment of human buccal recession. J. Periodontol. 1992; 63: 919-28.Muller H.P., Stahl M., Eger T. Failure of root coverage of shallow gingival recessions employing GTR and a bioresorbable membrane. Int. J. Periodontics Restorative Dent. 2001; 21: 171-81.Wang H-L., Bunyaratavej P., Labadie M. et al. Comparison of 2 techniques for treatment of gingival recession. J. Periodontol. 2001; 72: 1301-11.Harris R.J. A comparative study of root coverage obtained with guided tissue regeneration utilizing a bioabsorbable membrane versus the connective tissue with partial-thickness double pedicle graft. J. Periodontol. 1997; 68: 779-90.Sommar P., Pettersson S., Ness C. et al. Engineering three-dimensional cartilageand bone-like tissues using human dermal fibroblasts and macroporous gelatine microcarriers. J. Plast. Reconstr. Aesthet. Surg. 2010; 63(6): 1036-46.Rosa A.L., de Oliveira P.T., Beloti M.M. Macroporous scaffolds associated with cells to construct a hybrid biomaterial for bone tissue engineering. Expert. Rev. Med. Devices. 2008; 5(6): 719-28.McGuire M.K., Nunn M.E. Evaluation of the safety and efficacy of periodontal applications of a living tissue-engineered human fibroblast-derived dermal substitute. I. Comparison to the gingival autograft: a randomized controlled pilot study. J. Periodontol. 2005; 76(6): 867-80.Yamada K., Yamaura J., Katoh M. et al. Fabrication of cultured oral gingiva by tissue engineering techniques without materials of animal origin. J. Periodontol. 2006; 77(4): 672-7.McGuire M.K., Scheyer eT., Schupbach P. Growth factor-mediated treatment of recession defects: a randomized controlled trial and histologic and microcomputed tomography examination. J. Periodontol. 2009; 80: 550-64.McGuire M.K., Scheyer eT. A randomized, double-blind, placebo controlled study to determine the safety and efficacy of cultured and expanded autologous fibroblast injections for the treatment of interdental papillary insufficiency associated with the papilla priming procedure. J. Periodontol. 2007; 78: 4-17.Koseoglu S., Duran I., Saglam M et al. Efficacy of collagen membrane seeded with autologous gingival fibroblasts in gingival recession treatment: a randomized, controlled pilot study. J Periodontol. 2012 Dec 21. Epub ahead of print.Lorenz K., Sicker M., Schmelzer E. et al. Multilineage differentiation potential of human dermal skin-derived fibroblasts. Exp. Dermatol. 2008; 17(11): 925-32.Junker J.P., Sommar P., Skog M. et al. Adipogenic, chondrogenic and osteogenic differentiation of clonally derived human dermal fibroblasts. Cells Tiss. Organ. 2010; 191(2): 105-18.Киселева Е.В., Чермных Э.С., Воротеляк Е.А. и др. Сравнение дифференцировочных потенций фибробластоподобных клеток стромы костного мозга, жировой ткани, волосяного сосочка и фибробластов дермы человека. Цитология 2009; 51(1): 12-9.Alt E., Yan Y., Gehmert S. et al. Fibroblasts share mesenchymal phenotypes with stem cells, but lack their differentiation and colony-forming potential. Biol. Cell. 2011; 103(4): 197-208.Блинова М.И., Калмыкова Н.В., Юдинцева Н.М. и др. Использование культивируемых клеток кожи человека для лечения трофических язв. Информ. бюлл. «Клеточные культуры» 2006; 21: 33-44.Калмыкова Н.В., Блинова М.И., Юдинцева Н.М. и др. Эквивалент кожи и способ его получения. Патент РФ 2342164. 2006 Апр 05.Pittenger M.F., Mbalaviele G., Black M. et al. Primary mesenchymal cells. In: Koller M.R., Palsson B.O., Masters G.R.W., editors. Mesenchymal Stem Cells. Human Cell Culture. Kluver Acad. Publ.; 2001. p. 189-207.Chandrakasan G., Torchia D., Piez K. Preparation of intact monomeric collagen from rat tail tendon and skin and the structure of the nonhelical ends in solution. J. Biol. Chem. 1976; 251: 6062-67.Кухарева Л.В. Гидротермическое сокращение коллагеновых волокон как метод исследования их структуры. Цитология 2009; 51(3): 257-64.Kalkwarf K.L., Krejci R.F., Berry W.C. Jr. Chronic mucogingival defects in miniature swine. J. Periodontol. 1983; 54(2): 81-5.Tarnow D.P. Semilunar coronally repositioned flap. J. Clin. Periodontol. 1986; 13(3): 182-5.