Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12156810.23868/gc121568ArticlesСтатьиReview ArticleCharacterization and ex vivo expansion umbilical cord bloodhematopoietic stem and progenitor cellsХарактеристика и ex vivo экспансия гемопоэтических стволовых и прогениторных клеток пуповинной кровиUfimtcevaA. IУфимцеваА. ИKanovE. VКановЕ. В«Trans-Technologies Ltd», Saint-PetersburgООО «Транс-Технологии», Санкт-Петербург1512201274VOL 7, NO4 (2012)ТОМ 7, №4 (2012)212711012023Copyright ©; 2012, Eco-VectorCopyright ©; 2012, Эко-Вектор2012Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121568

Umbilical cord blood as an alternative source of hematopoietic stem and progenitor cells is widely used in clinical practice. Thereby it is necessary to characterize hematopoietic progenitors and to define among them the most «primitive» cells with the greatest repopulating potential. In addition absolute number of hematopoietic stem and progenitor cells is limited, so it is important to find ways of increasing their ex vivo. Currently several methods of in vitro and in vivo characterize hematopoietic progenitors and of ex vivo expansion of these cells have been developed. In this review we describe this methods, as well as problems with their translation to the clinical transplantation.

Пуповинная кровь, как один из альтернативных источников гемопоэтических стволовых и прогениторных клеток, в настоящее время активно применяется в клинической практике. В связи с этим необходимо четко охарактеризовать содержащуюся в ней популяцию гемопоэтических предшественников и обнаружить среди них клетки с наибольшей способностью к восстановлению кроветворения. Кроме того, абсолютное количество гемопоэтических стволовых и кроветворных клеток в пуповинной крови невелико, что заставляет искать способы их наращивания ex vivo. В настоящее время разработан ряд методов определения характеристик прогениторных клеток пуповинной крови in vitro и in vivo и их экспансии ex vivo. В данном обзоре рассмотрены эти методы, а также проблемы их адаптации для практики клинической трансплантологии.

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