Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12155110.23868/gc121551ArticlesСтатьиAge effects on angiogenic properties of adipose tissue mesenchymal stem cellsВлияние возраста на ангиогенные свойствамезенхимальных стволовых клеток жировой тканиEfimenko,A YuЕфименко,А Ю

M.V. Lomonosov Moscow State University, Moscow

Московский государственный университет им. М.В. Ломоносова, Москва

Starostina,E EСтаростина,Е Е

M.V. Lomonosov Moscow State University, Moscow

Московский государственный университет им. М.В. Ломоносова, Москва

Kalinina,N IКалинина,Н И

M.V. Lomonosov Moscow State University, Moscow

Московский государственный университет им. М.В. Ломоносова, Москва

ParfyenovaE VПарфеноваЕ В

M.V. Lomonosov Moscow State University, Moscow

Московский государственный университет им. М.В. Ломоносова, Москва

M.V. Lomonosov Moscow State University, MoscowМосковский государственный университет им. М.В. Ломоносова, Москва1509201163NO3 (2011)№3 (2011)485711012023Copyright ©; 2011, Eco-VectorCopyright ©; 2011, Эко-Вектор2011Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121551

Мезенхимальные стволовые клетки жировой ткани (МСК-ЖТ) способны стимулировать ангиогенез посред- ством продукции факторов роста и стабилизации растущих сосудов. МСК-ЖТ являются перспективным материалом для аутологичной клеточной терапии. Однако с возрас- том пациентов активность их МСК-ЖТ, а, следовательно, и эффективность клеточной терапии могут снижаться. Целью нашей работы было оценить ангиогенные свойства МСК-ЖТ при старении. Материал и методы. МСК-ЖТ были выделены из под- кожной жировой ткани мышей линии BalB/с возраста 1-2, 12, 18 и 24 мес., а также 12 доноров трех возрастных групп (12 (5; 12) лет, 45 (41; 45) лет и 65 (59; 76) лет). МСК-ЖТ 2-го пассажа помещали на 48 ч в условия 1% или 20% содержания кислорода. Оценивали способность клеток стимулировать ангиогенез in vitro и in vivo. Результаты. Способность МСК-ЖТ мышей стимулиро- вать формирование капилляроподобных структур in vitro и васкуляризацию подкожных имплантатов Матригеля in vivo с возрастом снижалась в среднем на 60%. Содержание мРНК сосудистого эндотелиального фактора роста (VEGF) и плацентарного фактора роста (PlGF) в этих клетках сни- жалось, а фактора роста гепатоцитов (HGF) и компонентов системы внеклеточного протеолиза (рецептор к урокиназе, металлопротеиназы 2 и 9 типов, ингибитор активатора плазминогена-1) - возрастало с возрастом животных. Сти- муляция экспрессии VEGF, PlGF и HGF в условиях гипоксии была выражена слабее в случае МСК-ЖТ старых живот- ных. Экспрессия PlGF и ангиопоэтина-1, а также секреция VEGF и HGF МСК-ЖТ человека отрицательно коррелирова- ли с возрастом донора. Таким образом, при старении способность МСК-ЖТ сти- мулировать ангиогенез снижается в результате подавления экспрессии ключевых ангиогенных факторов.

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