Genes & CellsGenes & Cells2313-18292500-2562Human Stem Cells Institute12149810.23868/gc121498Kompozitsionnye matritsy na osnove khitina i khitozana dlya kul'tivirovaniya kletok kozhi chelovekaPanarinE Fthe Institute of Macromolecular Compounds, RAS, Saint-Petersburg 2 the Institute of Cytology, RAS, Saint-Petersburg-Nud'gaL Athe Institute of Macromolecular Compounds, RAS, Saint-Petersburg-PetrovaV Athe Institute of Macromolecular Compounds, RAS, Saint-Petersburg-BochekA Mthe Institute of Macromolecular Compounds, RAS, Saint-Petersburg-GoffmanI Vthe Institute of Macromolecular Compounds, RAS, Saint-Petersburg-BaklaginaYu Gthe Institute of Macromolecular Compounds, RAS, Saint-Petersburg-SaprykinaN Nthe Institute of Macromolecular Compounds, RAS, Saint-Petersburg-BlinovaM Ithe Institute of Cytology, RAS, Saint-Petersburg-YudintsevaN Mthe Institute of Cytology, RAS, Saint-Petersburg-SpichkinaO Gthe Institute of Cytology, RAS, Saint-Petersburg-KukharevaL Vthe Institute of Cytology, RAS, Saint-Petersburg-SamusenkoI AA.M. Nikiforov National Center of Urgent and Radiation Medicine, EMERCOM of Russia, Saint-Petersburg-PinaevG Pthe Institute of Cytology, RAS, Saint-Petersburg-the Institute of Macromolecular Compounds, RAS, Saint-Petersburg 2 the Institute of Cytology, RAS, Saint-Petersburgthe Institute of Macromolecular Compounds, RAS, Saint-Petersburgthe Institute of Cytology, RAS, Saint-PetersburgA.M. Nikiforov National Center of Urgent and Radiation Medicine, EMERCOM of Russia, Saint-Petersburg1503201051657311012023Copyright © 2010, Eco-Vector2010A set of composite films based on chitosan or chitosan-collagen mixtures are developed to be used as the resorbable matrices for fibroblastes and keratinocytes cultivation, and optimal conditions of their formation are precised. 3D porous matrices are obtained to insure the simultaneous cultivation of both types of cells. The composite materials prepared by this method in forms of films and sponges were characterized and then tested as the matrices for cell cultivation to obtain the transplantats for wound healing. Both in vitro and in vivo tests of all materials under study have evidenced the absence of any toxic actions on the cultivated cells and on the tissues of organism. The matrices are stable in the conditions of cultivation and resorb completely in in vivo conditions in 10 days after the transplantation.polysaccharideschitinchitosancollagenspongemembranesfibroblastskeratinocytesculturingПолисахаридыхитинхитозанколлагенпленкигубкифибробластыкератиноцитыкультивирование[Панарин Е.Ф., Нудьга Л.А., Петрова В.А. и соавт. Матрицы для культивирования клеток кожи человека на основе природных полисахаридов - хитина и хитозана. Клеточная трансплантология и тканевая инженерия 2009; IVC3]: 42-6.][Terbojevich М., Corrado С, Cosani A., Marsano Е. Solution studies the chitin - lithium chloride - N,N-dimethylacetamide system. Carbohydr. Res. 1988; 180:73-86.][Chandrakasan G., Torchia D.A., Piez K.H.. Preparation of intact monomeric collagen from rat tail tendon and skin and the structure of the helical ends in solution. J. Biol. Chem. 1976; 251(19]: 6062-7.][Хилькин A.M., Шехтер А.Б., Истранов Л.П., Леменев В.Л. Коллаген и его применение в медицине. М.: Медицина 1976: 228.][Михайлов Г.М., Лебедева М.Ф., Нудьга Л.А. и др. Структура и механические свойства волокон из хитина, модифицированных эфирами целлюлозы. Материалы Пятой конф. «Новые перспективы в исследовании хитина и хитозана». Москва-Щелково 1999: 51.]