Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12148510.23868/gc121485ArticlesСтатьи3D culturing: from individual cells to blastemic tissue (Revisited the phenomenon of epithelial - mesenchymal plasticity)ЗD-культивирование: от отдельных клеток к регенерационной ткани (К вопросу о феномене эпителио-мезенхимальной пластичности)SaburinaI NСабуринаИ Н

УРАМН НИИ общей патологии и патофизиологии РАМН, Москва

RepinB CРепинB C

УРАМН НИИ общей патологии и патофизиологии РАМН, Москва

SaburinaI N

The RAMS Institute of General Pathology and Pathophysiology, Moscow

RepinVS

The RAMS Institute of General Pathology and Pathophysiology, Moscow

УРАМН НИИ общей патологии и патофизиологии РАМН, МоскваThe RAMS Institute of General Pathology and Pathophysiology, Moscow1506201052NO2 (2010)№2 (2010)758611012023Copyright ©; 2010, Eco-VectorCopyright ©; 2010, Эко-Вектор2010Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/121485

The phenomenon of epithelial - mesenchymal plasticity of multipotent mesenchymal stromal cells CMMSCsJ observed in 2D cultures has not been clarified yet. MMSC 3D culturing to model the patterns of epithelial cell layer formation (lamination] gives new opportunities. The review of literature and own findings enabled a suggestion that it is spontaneous formation of MMSC spheroids that induces generation of blastemic tissue in fetal and adult organs when damaged. The feasibility of reprogramming dense MMSC spheroids into neuroectoderm and primitive entoderm has been demonstrated.

Феномену эпителио-мезенхимальной пластичности муль-типотентных мезенхимальных стромальных клеток CMMCKJ, наблюдаемому в 2D культурах, до сих пор не найдено удовлетворительного объяснения. Новые возможности открывает 3D культивирование ММСК, которое моделирует закономерности образования слоев эпителиальных клеток Сламинацию]. Проведенный анализ собственных и литературных данных позволил предположить, что именно спонтанное формирование ММСК-сфероидов индуцирует образование регенерационной («бластемной»] ткани в фетальных и взрослых органах при повреждении. Продемонстрирована возможность репрограм-мирования плотных сфероидов ММСК в нейроэктодерму и примитивную энтодерму.

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