Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12059710.23868/gc120597ArticlesСтатьиResearch ArticlePlakophilin-2 gene knockout using CRISPR/Cas9 systemНОКАУТ ГЕНА ПЛАКОФИЛЛИНА-2 ПРИ ПОМОЩИ СИСТЕМЫ CRISPR/CAS9KhudiakovA. AХудяковА. АKostinaDA. AКостинаД. АKostarevaA. AКостареваА. АMalashichevaA. BМалашичеваА. Бamalashicheva@gmail.comFederal V.A. Almazov North West Medical Research CentreСеверо-Западный федеральный медицинский исследовательский центр им. В.А. АлмазоваStaint-Petersburg State UniversityСанкт-Петербургский государственный университет15062016112VOL 11, NO2 (2016)ТОМ 11, №2 (2016)959905012023Copyright ©; 2016, Eco-VectorCopyright ©; 2016, Эко-Вектор2016Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/120597

CRISPR/Cas9 technology opens up broad prospects for genome manipulations. Generation of gene knockout cell lines is one of the applications of this system. This approach allows to reveal previously unknown gene functions and to reproduce the effect of pathogenic mutations. The purpose of this study was to generate plakofilin-2 gene knockout system and assess its suitability for the study of the functional effect of this knockout.

Использование системы CRISPR/Cas9 открывает широкие перспективы для манипуляций с геномом. Одним из применений этой системы является создание клеточных линий, нокаутных по тому или иному гену. Этот подход позволяет обнаружить неизвестные ранее функции гена и воспроизвести эффект патогенных мутаций. Целью настоящего исследование являлось создание системы для нокаута гена плакофиллина-2 и оценка её пригодности для изучения функционального эффекта нокаута.

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