Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12058910.23868/gc120589ArticlesСтатьиResearch ArticleLong noncoding RNA expression pattern in the X inactivation center of rodentsПРОФИЛИ ЭКСПРЕССИИ НЕТРАНСЛИРУЕМЫХ РНК В ЦЕНТРЕ ИНАКТИВАЦИИ У МЫШЕВИДНЫХ ГРЫЗУНОВElisaphenkoE. AЕлисафенкоЕ. Аantares@bionet.nsc.ruShevchenkoA. IШевченкоА. Иantares@bionet.nsc.ruZakianS. MЗакиянС. Мantares@bionet.nsc.ruFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of SciencesФГБНУ «Федеральный исследовательский центр Институт цитологии и генетики СО РАН»Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of SciencesФГБНУ «Институтхимической биологии и фундаментальной медицины СО РАН»State Research Institute of Circulation Pathology, Ministry of Healthcare of the Russian FederationФГБУ «Новосибирский научно-исследовательский институт патологии кровообращения имени академика Е.Н. Мешалкина» Министерства здравоохранения РФNational Research University Novosibirsk State UniversityНовосибирский национальный исследовательский государственный университет15062016112VOL 11, NO2 (2016)ТОМ 11, №2 (2016)828605012023Copyright ©; 2016, Eco-VectorCopyright ©; 2016, Эко-Вектор2016Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/120589

Transcriptome analysis of several cell lines with different differentiation degree has been carried out in three rodent species - voles, rats, and mice. As a result, expression profiles of X-inactivation center genes have been generated. In mice, new data on how expression of key genes of X-inactivation center, Xist and Tsix, changes during ontogenesis have been obtained. New types of transcripts have been revealed. In voles, Xist expression was observed in all 8 cell lines examined. Interestingly, main vole Xist transcript had 7 exons as those of rats and mice. In addition, Xist antisense transcript, Tsix, was expressed in 4 vole cell lines and was presented by both spliced and unspliced variants. Expression between the Enox (Jpx) and Xist genes has been revealed in three vole cell lines. Additional variants of Tsix transcripts were observed when studied expression profiles of several rat cell lines. The variants were not present in mice and voles. Compared to mice and voles, Tsix expression level in rats was several times higher than that of Xist.

Для трех видов грызунов полевок, крысы и мыши проведен анализ транскриптомов нескольких клеточных линий, различающихся степенью дифференцировки, по результатам которого построены профили экспрессии генов в центре инактивации Х-хромосомы. Для мыши получены новые данные о том, как изменяется экспрессия ключевых генов центра инактивации Xist и Tsix в онтогенезе. Выявлены новые формы транскриптов. У полевок во всех 8 исследованных линиях клеток наблюдается экспрессия гена Xist. Интересно отметить, что преобладающий транскрипт гена Xist полевок, также как Xist крысы и мыши, имеет 7 экзонов. Кроме того, в четырех линиях клеток полевок, экспрессируется антисмысловой транскрипт - Tsix, который представлен как сплайсированными, так и несплайсированными вариантами. В трех линиях клеток у полевок выявляется экспрессия между генами Enox (Jpx) и Xist. При исследовании профиля экспрессии нескольких клеточных линий крысы были выявлены дополнительные варианты транскриптов гена Tsix, которых нет у мыши и полевки. Уровень экспрессии гена Tsix у крысы, в отличие от мыши и полевки, в разы превышает экспрессию Xist.

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