Genes & CellsGenes & CellsГены и КлеткиGenes and Cells2313-18292500-2562Human Stem Cells Institute12057810.23868/gc120578ArticlesСтатьиResearch ArticleCytokines and growth factors genes expression after subtotal liver resection in ratsЭкспрессия генов цитокинов и факторов роста в печени после субтотальной резекции у крысElchaninovA. VЕльчаниновА. ВFatkhudinovT. KhФатхудиновТ. Хfatkhudinov@gmail.comUsmanN. YУсманН. ЮArutyunyanI. VАрутюнянИ. ВMakarovA. VМакаровА. ВKananykhinaE. YКананыхинаЕ. ЮRaimovaE. ShРаимоваЭ. ШBolshakovaG. BБольшаковаГ. БSukhikhG. TСухихГ. ТResearch Center for Obstetrics, Gynecology and PerinatologyНаучный центр акушерства, гинекологии и перинатологии им. ак. В.И. КулаковаScientific Research Institute of Human MorphologyНаучно-исследовательский институт морфологии человекаN.I. Pirogov Russian National Research Medical UniversityРоссийский национальный исследовательский медицинский университет им. Н.И. ПироговаN.I. Pirogov Russian National Research Medical UniversityНаучный центр акушерства, гинекологии и перинатологии им. ак. В.И. КулаковаResearch Center for Obstetrics, Gynecology and PerinatologyНаучно-исследовательский институт морфологии человекаScientific Research Institute of Human MorphologyРоссийский национальный исследовательский медицинский университет им. Н.И. ПироговаN.I. Pirogov Russian National Research Medical UniversityНаучно-исследовательский институт морфологии человекаScientific Research Institute of Human MorphologyНаучный центр акушерства, гинекологии и перинатологии им. ак. В.И. КулаковаResearch Center for Obstetrics, Gynecology and Perinatology15032016111VOL 11, NO1 (2016)ТОМ 11, №1 (2016)616705012023Copyright ©; 2016, Eco-VectorCopyright ©; 2016, Эко-Вектор2016Eco-VectorЭко-Векторhttps://genescells.ru/2313-1829/article/view/120578

Liver regeneration after 70% resection is one of the most studied models of tissue regeneration Small residual liver volume after massive hepatectomy or partial liver transplantation is a major cause of subsequent liver dysfunction known in oncology and transplantology as small-for-size syndrome The aim of this study was to evaluate the influence of 80% liver resection on cytokines and growth gens expression in rat Male Wistar rats were subjected to 80% and sacrificed at different times after surgery. Untreated animals served as controls Serum and liver samples were obtained to investigate liver function, cytokines and growth factors genes expression: il1b, il6, il10, tnfa, tweak, mmp9, fgf2, tgfb, hgf, vegf, sdfa, ang with quantitative RT-PCR. It was revealed two phases increased expression of genes that correspond to the first and second peak hepatocytes proliferation The first phase was characterized by increased expression mmp9, il6, il10, fgf2, tgfb. The second phase was characterized by increased expression of hgf, tnfa, tweak, il1b. Furthermore, we have found increased expression of the transcription factor SOX9 gene, and the gene encoding TWEAK, indicating a possible role for resident progenitor cells in the regenerating liver of rats after subtotal resection In our opinion the two phases of increased gene expression during liver regeneration in rats after subtotal resection are associated with two waves of hepatocyte proliferation, and the presence of growth factors reserves in the liver.

Регенерации печени млекопитающих после 70% резекции посвящено большое количество исследований. Субтотальная резекция печени часто приводит к печеночной недостаточности Подобное состояние в онкологии и трансплантологии известно как синдром малого остатка органа. Цель работы - изучить динамику экспрессии генов основных сигнальных молекул при регенерации печени после субтотальной резекции у крыс. На самцах крыс породы Вистар воспроизведена модель регенерации печени после субтотальной резекции С помощью полимеразной цепной реакции в режиме реального времени изучена экспрессия генов il1b, il6, il10, tnfa, tweak, mmp9, fgf2, tgfb, hgf, vegf, sdfa, ang. Выявлено две фазы повышенной экспрессии генов, которые соответствуют первому и второму пикам пролиферации гепатоцитов Для первой фазы характерно повышение экспрессии mmp9, il6, il10, fgf2, tgfb. Во вторую фазу была повышена экспрессия hgf, tnfa, tweak, il1b. Кроме того, мы обнаружили повышение экспрессии гена фактора транскрипции SOX9, а также гена, кодирующего TWEAK, что указывает на возможную роль резидентных прогениторных клеток в регенерации печени крыс после такой травмы На наш взгляд, наличие двух фаз повышенной экспрессии генов при регенерации печени крыс после субтотальной резекции связано с двумя волнами пролиферации гепатоцитов, а также с наличием запасов факторов роста в печени.

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