Genes & CellsGenes & Cells2313-18292500-2562Human Stem Cells Institute12054410.23868/gc120544Research ArticleEfficiency of autofibroblasts in surgical treatment of parodontitisGrudyanovA. I-ZorinV. L-PereverzevR. V-ZorinaA. I-BozoI. Y-Central Research Institute of Dentistry and maxillofacial surgery of MH RussiaHuman Stem Cells InstituteA.I. Evdokimov Moscow State University of Medicine and Dentistry1510201383727705012023Copyright © 2013, Eco-Vector2013The destructive forms of periodontal disease have a high prevalence. Our study was aimed to evaluate the effectiveness of tissue-engineering osteoplastic material in treatment of patients with periodontal pathology. We have obtained autologous gingival fibroblasts of patients, combined with the hydroxyapatite carriage and transplanted on a standard surgical treatment of periodontitis destructive forms. As a control we used the material without cells implanted into periodontal defects of other affected teeth of the same patients. We have shown the better effect of tissue-engineering osteoplastic material regarding the periodontal pockets depth, but significant difference in bone regeneration and other indicators has not been revealed. Thus, a continuation of researches is required to modify the technology of tissueengineering osteoplastic material creation and to choice the more optimal carrier for gingival fibroblasts.gingival fibroblaststissue-engineering osteoplastic materialperiodontal diseaseclinical studyфибробласты слизистой оболочкитканеинженерный остеопластический материалпародонтитклиническое исследование[Безрукова И.В. Быстропрогрессирующий пародонтит. М.: Медицинская книга; 2004.][Григорьян А.С., Грудянов А.И., Фролова О.А. и др. Видовой состав анаэробной микрофлоры пародонтального кармана в зависимости от стадии пародонтита. Стоматология 2009; 4: 43-7.][Грудянов А.И. Заболевания пародонта. М.: Медицинское информационное агентство; 2009.][Petersen P.E., Ogawa H. Strengthening the prevention of periodontal disease: the WHO approach. Periodontol 2005; 76: 2187-93.][Григорьян А.С., Грудянов А.И., Рабухина Н.А. Болезни паро-донта: патогенез, диагностика, лечение. М.: Медицинское информационное агентство; 2004.][Bowers G.M., Chadroff B., Carnevale R. et al. Histologic evaluation of new attachment apparatus formation in humans. Part III. Periodontol. 1989; 60(12): 683-93.][Cortellini P, Bowers G. Periodontal regeneration of intrabony defects: An evidence based treatment approach. Int. J. Periodontol. Rest. Dent. 1995; 15:128-45.][Гололобов В.Г., Дулаев А.К., Деев Р.В. и д.р. Морфофункциональная организация, реактивность и регенерация костной ткани. СПб: 2006; 47 с.][Зорин В.Л., Зорина А.И., Воложин Г.А. и др. Изучение фенотипического профиля и остеогенных свойств фибробластов десны. Саратовский научно-медицинский журнал 2013; 9(3): 393-7.][Lorenz K., Sicker M., Schmelzer E. et al. Multilineage differentiation potential of human dermal skin-derived fibroblasts. Exp. Dermatol. 2008; 17(11): 925-32][Mostafa N.Z., Uludag H., Varkey M. et al. In vitro osteogenic induction of human gingival fibroblasts for bone regeneration. Open Dent J. 2011;5:139-45.][Huang G.T., Gronthos S., Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J. Dent. Res. 2009; 88(9): 792806.][Guo W., Chen L., Gong K. et al. Heterogeneous dental follicle cells and the regeneration of complex periodontal tissues. Tissue Eng. Part A. 2012; 18(5-6): 459-70.]