Perculiarities of physiologic and reparative osteogenesis following the transfusion of bone marrow mononuclear cells

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Abstract

Physiologic and reparative regeneration of bone tissue after transfusion of bone marrow GFP-positive mononuclear cells of mice C57B1 /6-TgN(ACTbGFP)1 Osb was studied in C57B1/6 mice exposed to radiation. It is shown that transplanted cells are able to differentiate into osteoblasts and osteocytes in both physiologic and reparative osteogenesis, these cells having priority to proliferation and differentiation in the recipient exposed to radiation. The GFP-positive osteocytes number within the substance reached 31±8%.

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About the authors

R. V. Deev

Kirov Military Medical Academy

Author for correspondence.
Email: redaktor@celltranspl.ru
Russian Federation, Saint-Petersburg

N. V. Tsupkina

RAS Institution of Cytology (Cellular Cultures Department)

Email: redaktor@celltranspl.ru
Russian Federation, Saint-Petersburg

V. S. Sergeev

Kirov Military Medical Academy

Email: redaktor@celltranspl.ru
Russian Federation, Saint-Petersburg

V. B. Serikov

Children’s Hospital Oakland Research Institute

Email: redaktor@celltranspl.ru
United States, Oakland, CA 94609

V. G. Gololobov

Kirov Military Medical Academy

Email: redaktor@celltranspl.ru
Russian Federation, Saint-Petersburg

G. P. Pinaev

RAS Institution of Cytology (Cellular Cultures Department)

Email: redaktor@celltranspl.ru
Russian Federation, Saint-Petersburg

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Supplementary files

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1. Fig. 1. Scheme of the experiment

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2. Fig. 2. Osteocytes in the compact substance of the bone tissue of the tibial diaphysis of the mouse of the first group (physiological osteohistogenesis)

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3. Fig. 3. Donor (GFP-positive] osteocytes in the compact substance of the bone tissue of the tibial diaphysis of the mouse of the first group (physiological osteohistogenesis)

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4. Fig. 4. The basic multicellular unit remodeling the bone tissue of the tibial diaphysis, an animal of the first group: a — resorption cone; B — reversion zone; c — closing cone.

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5. Fig. 5. A basic multicellular unit remodeling the bone tissue of the tibial diaphysis, an animal of the first group

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6. Fig. 6. Bone-cartilaginous trabecula as part of the regenerate in animals of the second group, constructed from GFP-positive cells

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