The effect of severe polytrauma on the migration of hematopoietic stem cells in mice

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It has been shown that hematopoietic stem cells (SCCS) are involved in the physiological and reparative regeneration of non-hematopoietic tissues and organs through transdifferentiation and/or cell fusion. In this regard, it seems interesting to evaluate the kinetics of bone marrow CCM in severe trauma. Three experimental models were used in the study: injury to mice followed by uneven irradiation in lethal doses with 1/2 shin screening (A), irradiation of mice in lethal doses followed by transplantation of bone marrow cells from injured mice (B), injury to mice followed by irradiation in sublethal doses (C). The migration of CCM was assessed by registering hematopoietic colonies growing on the spleen of irradiated mice from endo- or exogenous SCCS. In experimental models A and B, it was shown that a significantly larger number of endocolonies are formed in the spleens of injured mice in comparison with mice of control groups. In the experimental model B, it was shown that the bone marrow of injured mice contains a significantly smaller number of CCM capable of migration. Based on the results obtained, it was concluded that severe polytrauma induces CCM migration in mice.

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V. N. Alexandrov

S.M. Kirov Military Medical Academy

Author for correspondence.
Russian Federation, Saint-Petersburg

V. S. Sergeev

S.M. Kirov Military Medical Academy

Russian Federation, Saint-Petersburg


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Supplementary files

Supplementary Files
1. Dynamic balance between the CCM of bone marrow, blood and non-hematopoietic tissues and organs in norm and pathology

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