Cultivation properties of human embryonic stem cells in the absence of serum and feeder layer
- Authors: Philonenko E.S.1, Kamnev A.N.1, Zautorov V.G.1, Shutova M.V.1, Tskhovrebova L.V.1, Bogomazova A.N.1, Kiselev S.L.1, Lagarkova M.A.1
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Affiliations:
- Vavilov lnstitute of general genetics
- Issue: Vol 3, No 3 (2008)
- Pages: 31-33
- Section: Original Study Articles
- URL: https://genescells.ru/2313-1829/article/view/159403
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Abstract
Cultivation conditions for five human embryonic stem cell lines (hESM01, hESM02, hESM03, hESM04, HES-9) were switched from serum replacement containing medium with mouse embryonic fibroblasts as a feeder to two commercialy available media (mTESR1 by StemCell Technologies, Canada and StemPro by lnvitrogen, USA). Cell lines were maintained in these media for more than 20 passages. Matrigel (BD Biosciences, USA) was used as a substrate. hESCs cultivated in defined media and without animal derived components retain their pluripotency and differentiation ability. Changes in morphology of feeder-free hESC colonies were observed. Feeder-free hESC cultivation gives possibility to get larger amount of cells due to increase of proliferative activity.
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About the authors
E. S. Philonenko
Vavilov lnstitute of general genetics
Author for correspondence.
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
A. N. Kamnev
Vavilov lnstitute of general genetics
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
V. G. Zautorov
Vavilov lnstitute of general genetics
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
M. V. Shutova
Vavilov lnstitute of general genetics
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
L. V. Tskhovrebova
Vavilov lnstitute of general genetics
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
A. N. Bogomazova
Vavilov lnstitute of general genetics
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
S. L. Kiselev
Vavilov lnstitute of general genetics
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
M. A. Lagarkova
Vavilov lnstitute of general genetics
Email: bozo.ilya@gmail.com
Russian Federation, Moscow
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