Quantitative accounting of CD34+ hematopoietic stem cells in whole cord blood
- Authors: Kurtova A.V.1, Zueva E.E.1
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Affiliations:
- Laboratory Diagnostics Center of St. Petersburg State Medical University named after Academician IL. Pavlov
- Issue: No 1 (2006)
- Pages: 66-71
- Section: Original Study Articles
- URL: https://genescells.ru/2313-1829/article/view/126445
- DOI: https://doi.org/10.23868/gc126445
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Abstract
Umbilical cord blood is an alternative source of hematopoietic stem cells to bone marrow and mobilized peripheral blood [HSC]. The population of CD34" cells in umbilical cord blood is small, which creates certain difficulties in carrying out quantitative accounting of HSC. This article discusses some practical aspects of determining CD34" HSC in whole cord blood. A preliminary assessment of the amount of HSC in the samples before the start of preparation for long-term storage may become an additional selection criterion when receiving cord blood in non-commercial anonymous banks.
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About the authors
A. V. Kurtova
Laboratory Diagnostics Center of St. Petersburg State Medical University named after Academician IL. Pavlov
Author for correspondence.
Email: redaktor@celltranspl.ru
Laboratory of Clinical Immunology and Molecular Diagnostics
Russian Federation, Saint-PetersburgE. E. Zueva
Laboratory Diagnostics Center of St. Petersburg State Medical University named after Academician IL. Pavlov
Email: redaktor@celltranspl.ru
Laboratory of Clinical Immunology and Molecular Diagnostics
Russian Federation, Saint-PetersburgReferences
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Supplementary files
Supplementary Files
Action
1.
Fig. 1. Two-dimensional histogram of light scattering parameters of a sample of whole cord blood. The unusual pattern of distribution of populations of lymphocytes and monocytes draws attention. The affiliation of the clusters to these populations was determined by analyzing the CD45/SSC histogram
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2.
Fig. 2. The sequence of creating logical constraints during the quantitative accounting of GSK
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3.
Fig. 3. Sample N. Two-dimensional histogram of light scattering parameters. Imposing a logical restriction on leukocytes on the CD45/CD34 histogram in this case does not allow unambiguously identifying the HSC population.
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4.
Fig. 4. Sample N. Two-dimensional histogram of light scattering parameters. The imposition of a logical restriction on mononuclears on the CD45/CD34 histogram makes it possible to identify the target population, which is confirmed by the quantitative coincidence of HSCs in gates R2 and R4
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5.
Fig. 5. Identification of two populations of CD34+ cells in a sample of whole cord blood prepared according to the staining-lysis protocol. Reverse gating confirmed that both populations belong to the lymphocyte region
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6.
Fig. 6. A sample of whole cord blood with a very low content CD34+ HSC cells are small in number and do not form clusters, which indicates the absence of clonogenic activity in them
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