Effect of pCMV-VEGF165 plasmides on the reparation of full-layer skin wound in experiment
- Authors: Shestakova V.G1, Banin V.V2, Deev R.V3, Bazhenov D.V1
-
Affiliations:
- Tver State Medical University of Russia
- A.I. Evdokimov Moscow State University of Medicine and Dentistry
- I.I. Mechnikov North-Western State Medical University
- Issue: Vol 14, No 4 (2019)
- Pages: 25-28
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/122191
- DOI: https://doi.org/10.23868/201912028
- ID: 122191
Cite item
Abstract
The study of the regeneration of a full-layer skin wound under conditions of stimulated angiogenesis was carried out by paravulnare injection of a solution containing the plasmid pCMV-VEGF165. The inflammatory reaction in the experimental group of rats was more intense and already on day 7, the leukocyte shaft in the control was 1.3 times wider, and on day 14 in the experimental group, this indicator was 2.2 times less than the control figures. The indicators characterizing the development of granulation tissue on day 7, in the second group, 1.3 prevail over the control values, after 14 days, due to the maturation of young connective tissue - 1.1 times. The greatest differences in the comparison groups were found in relation to the degree of epithelialization of the defect. The epithelium thickness at the border is 1.4 times the control, the epithelial layer thickness is 1.7 times, and the length is 1.5 times. Compared with the control values, the number of vessels in the experimental group on day 7 were 2.3 times higher, on day 14 - 1.8 times and on day 21 - 1.7 times. The healing of the full-thickness wound of the skin in the experimental group proceeded more intensively compared with the control, and epithelization was completed 2 days earlier. In addition, stimulation of angiogenesis led to the formation of an organ-specific regenerate practically indistinguishable from intact skin.
Full Text

About the authors
V. G Shestakova
Tver State Medical University of Russia
Email: shestvg@mail.ru
Tver, Russia
V. V Banin
A.I. Evdokimov Moscow State University of Medicine and Dentistry
Email: shestvg@mail.ru
Moscow, Russia
R. V Deev
I.I. Mechnikov North-Western State Medical University
Email: shestvg@mail.ru
Saint Petersburg, Russia
D. V Bazhenov
Tver State Medical University of Russia
Email: shestvg@mail.ru
Tver, Russia
References
- Алексеева Н.Т., Никитюк Д.Б., Клочкова С.В. Аналитическая морфология репаративной регенерации в коже под действием различных региональных факторов. Журнал анатомии и гистопатологии 2015; 4(1): 26-38.
- Целуйко С.С., Кушнарев В.А. Регенеративная биомедицина: достижения и перспективы. Амурский медицинский журнал 2016; 1: 7-15.
- Семченко В.В., Барашкова С.Ф., Ноздрин В.Н. и др. Гистологическая техника: учебное пособие. 3-е изд. Омск-Орел: Омская областная типография; 2006. с. 290.
- Борздова Т.В. Основы статистического анализа и обработка данных с применением Microsoft Excel: учебное пособие. Минск: ГИУСТ БГУ; 2011. с. 109.
- Козлов В.И. Капилляроскопия в клинической практике. Монография. Москва: Практическая Медицина; 2015. с. 232.
- Silvestre J.S., Tamarat R. Ebrahimian T.G., Le-Roux A. et al. Vascular endothelial growth factor-B promotes in vivo angiogenesis. Circulation Research 2003; 93: 114-23.
- Шестакова В.Г., Банин В.В., Баженов Д.В. Особенности новообразования грануляционной ткани в полнослойной хирургической ране при стимуляции ангиогенеза «Неоваскулгеном». Журнал анатомии и гистопатологии 2015; 4(3): 140.
- Банин В.В. Роль перицитов в механизме новообразования сосудов регенерирующей соединительной ткани. Морфология 2004; 125(1): 45-50.
- Hudlicka O., Tyler K.R. Angiogenesis. The growth of the vascular system. London; Orlando, FL: Academic Press; 1986. р. 131.
