The first clinical experience of direct gene therapy using VEGF and bFGF in treatmentpatients with critical lower limb ischemia
- Authors: Plotnikov MV1,2,3, Rizvanov AA1,4,3, Masgutov RF1,4,5,3, Mavlikeev MO5, Salafutdinov II1,4,3, Gazizov IM5, Romanova YD4, Shamsutdinova II1, Bogov AA1,5,3, Maksimov AV1,2,3
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Affiliations:
- Republican Clinical Hospital, Kazan
- Kazan State Medical Academy, Kazan
- Kazan (Volga Region) Federal University, Kazan
- Kazan State Medical University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 180-184
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121717
- DOI: https://doi.org/10.23868/gc121717
- ID: 121717
Cite item
Abstract
successful treatment of distal form of peripheral artery
disease of the lower extremity with symptoms of critical
ischemia in a 60 years old patient.
Intramuscular injection of dual expression plasmid, encoding
vascular endothelial growth factor VEGF and basic fibroblast
growth factor FGF2, was performed to the affected lower
extremity. The effect of treatment was evaluated by functional
tests: measurement of ankle-brachial index, treadmill test, the
recovery time, shoulder-ankle index after strain and temporary
occlusion. Performed immunohistochemical examination of
biopsy samples of the affected lower extremity muscles.
About the authors
M V Plotnikov
Republican Clinical Hospital, Kazan;Kazan State Medical Academy, Kazan;Republican Clinical Hospital, Kazan;Kazan State Medical Academy, Kazan;
A A Rizvanov
Republican Clinical Hospital, Kazan;Kazan (Volga Region) Federal University, Kazan;Republican Clinical Hospital, Kazan;Kazan (Volga Region) Federal University, Kazan;
R F Masgutov
Republican Clinical Hospital, Kazan;Kazan (Volga Region) Federal University, Kazan;Kazan State Medical University, Kazan;Republican Clinical Hospital, Kazan;Kazan (Volga Region) Federal University, Kazan;Kazan State Medical University, Kazan;
M O Mavlikeev
Kazan State Medical University, KazanKazan State Medical University, Kazan
I I Salafutdinov
Republican Clinical Hospital, Kazan;Kazan (Volga Region) Federal University, Kazan;Republican Clinical Hospital, Kazan;Kazan (Volga Region) Federal University, Kazan;
I M Gazizov
Kazan State Medical University, KazanKazan State Medical University, Kazan
Y D Romanova
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
I I Shamsutdinova
Republican Clinical Hospital, KazanRepublican Clinical Hospital, Kazan
A A Bogov
Republican Clinical Hospital, Kazan;Kazan State Medical University, Kazan;Republican Clinical Hospital, Kazan;Kazan State Medical University, Kazan;
A V Maksimov
Republican Clinical Hospital, Kazan;Kazan State Medical Academy, Kazan;Republican Clinical Hospital, Kazan;Kazan State Medical Academy, Kazan;
References
- Савельев B.C., Кошкин В.М. Критическая ишемия нижних конечностей. М.: Медицина; 1997.
- Criqui M.H., Fronek A., Barrett-Connor E. et al. The prevalence of peripheral arterial disease in a defined population. Circulation 1985; 71(3): 510-5.
- Hiatt W.R., Hoag S., Hamman R.F. Effect of diagnostic criteria on the prevalence of peripheral arterial disease. The San Luis Valley Diabetes Study. Circulation 1995; 1; 91(5): 1472-9.
- Selvin E., Erlinger T.P. Prevalence of and risk factors for peripheral arterial disease in the United States: results from the National Health and Nutrition Examination Survey, 1999-2000. Circulation 2004; 110(6): 738-43.
- Muluk S.C., Muluk V.S., Kelley M.E. et al. Outcome events in patients with claudication: A 15-year study in 2777 patients. J. Vasc. Surg. 2001; 33: 251-8.
- Комаров А.Л, Панченко Е.Л., Деев А.Д. и др. Течение пере- межающейся хромоты и прогноз больных атеросклеротическим поражением артерий нижних конечностей. Анализ результатов про- спективного наблюдения. Ангиология и сосудистая хирургия 2000; 6(2): 9-18. 7. The Vascular Surgical Society of Great Britain and Ireland. Critical limb ischemia: management and outcome. Report of a national servey. Eur. J. Vas. Endovasc. Surg. 1995; 10: 108-13.
- Hirsch A.T., Haskal Z.J., Hertzer N.R. et al. ACC/AHA 2005 Guidelines for the Management of Patients with Peripheral Arterial Disease (Lower Extremity, Renal, Mesenteric, and Abdominal Aortic):A Collaborative Report from the American Association for VascularSurgery/Society for Vascular Surgery, Society for CardiovascularAngiography and Interventions, Society for Vascular Medicine andBiology, Society of Interventional Radiology, and the ACC/AHA TaskForce on Practice Guidelines (Writing Committee to Develop Guidelinesfor the Management of Patients With Peripheral Arterial Disease). J.Am. Coll. Cardiol. 2006; 47:1-192.
- Казанчан П.О., Попов В.А., Дебелый Ю.В. и др. Хирургиче- ская реваскуляризация при критической ишемии. Ангиология и со- судистая хирургия 2000; 3(32): 5.
- Деев Р.В., Григорян А.С., Потапов И.В. и соавт. Мировой опыт и тенденции генной терапии ишемических заболеваний. Ан- гиология и сосудистая хирургия 2011; 17 (2): 145-54.
- Хорев Н.Г., Елыкомов В.А., Залозный Д.А. Терапевтический клеточный ангиогенез в лечении заболеваний периферических ар- терий. Ангиология и сосудистая хирургия 2011; 17(2): 36-44.
- Салафутдинов И.И., Шафигуллина А.К., Ялвач М.Э. и соавт. Эффект одновременной экспрессии различных изоформ фактора роста эндотелия сосудов VEGF и основного фактора роста фибро- бластов FGF2 на пролиферацию эндотелиальных клеток пупочной вены человека HUVEC. Клеточная Трансплантология и Тканевая Ин- женерия 2010; 5(2): 62-7.
- Norrby K., Ridell B. Tumour-type-specific capillary endothelial cell stainability in malignant B-cell lymphomas using antibodies against CD31, CD34 and Factor VIII. APMIS 2003; 111: 483-9.
- Asahara T., Murohara T., Sullivan A. et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science 1997; 275:964-7.
- Швальб П.Г., Гавриленко А.В., Калинин Р.Е. и др. Эффек- тивность и безопасность применения препарата «Неоваскулген» в комплексной терапии пациентов с хронической ишемией нижних ко- нечностей (IIb-III фаза клинических испытаний). Клеточная Транс- плантология и Тканевая Инженерия 2011; 6(3): 76-83.
