Comparative study of an activity of rat spinal ganglion cells and PC12 cells on the surfacesmodified with bioadhesive polymers
- Authors: Yakunina, LD1, Kurbanov, RA1, Bondar, OV1, Abdullin TI1
-
Affiliations:
- Kazan (Volga Region) Federal University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 173-176
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121709
- DOI: https://doi.org/10.23868/gc121709
- ID: 121709
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Abstract
We studied the adsorption of bioadhesive polymers
(polyornithine, gelatin, laminin) on polystyrene surface
by the use of dynamic light scattering. The contribution
of biopolymers to resulting zeta potential of the modified
surface was assessed. PC12 cells do not exhibit selective
adhesion in the presence of foetal bovine serum. Polystyrene
with adsorbed polyornithine promotes primary adhesion
of PC12 cells cultured in serum-free medium with nerve
growth factor. Subsequently adsorbed laminin induces
spreading and differentiation of the cells into neuronal
direction. Primary neurons isolated from rat spinal ganglion
adhere preferentially on the polyornithine-modified surface.
On the polyornithine-laminin surface neurons intensively
form neuritis that correlates with proliferation of glial
cells positive for S100 protein. The results show that
PC12 cells and primary neurons exhibit similar response to
surface material with the latter cells being more sensitive
to this factor. Isolated cell culture can be used to study the
relationship between neurite outgrowth and Schwann cells
proliferation on different biomaterials.
(polyornithine, gelatin, laminin) on polystyrene surface
by the use of dynamic light scattering. The contribution
of biopolymers to resulting zeta potential of the modified
surface was assessed. PC12 cells do not exhibit selective
adhesion in the presence of foetal bovine serum. Polystyrene
with adsorbed polyornithine promotes primary adhesion
of PC12 cells cultured in serum-free medium with nerve
growth factor. Subsequently adsorbed laminin induces
spreading and differentiation of the cells into neuronal
direction. Primary neurons isolated from rat spinal ganglion
adhere preferentially on the polyornithine-modified surface.
On the polyornithine-laminin surface neurons intensively
form neuritis that correlates with proliferation of glial
cells positive for S100 protein. The results show that
PC12 cells and primary neurons exhibit similar response to
surface material with the latter cells being more sensitive
to this factor. Isolated cell culture can be used to study the
relationship between neurite outgrowth and Schwann cells
proliferation on different biomaterials.
About the authors
L D Yakunina,
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
R A Kurbanov,
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
O V Bondar,
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
T I Abdullin
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
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