Transplanted Hepatic Stellate Cells participate in liver regeneration after partial hepatectomywithout risk of hepatic fibrosis
- Authors: Shafigullina AK1, Gumerova AA1, Trondin AA1, Titova MA1, Gazizov IM1, Burganova GR1, Kaligin MS1, Andreeva DI1, Rizvanov AA2, Muhamedov AR1, Kiassov AP1
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Affiliations:
- Kazan State Medical University, Kazan
- Kazan Federal (Volga Region) University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 169-172
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121700
- DOI: https://doi.org/10.23868/gc121700
- ID: 121700
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Abstract
stem cells candidates in the liver. The aim of our work was to
study the probability of hepatic stellate cells transplantation
to rats after partial hepatectomy, their further homing, the
ways of differentiation and hepatocytes repopulation in the
recipient liver. For this reason fresh isolated rat`s hepatic
stellate cells were transplanted into portal vein of intact rats
(control group) and rats immediately after partial hepatectomy
(experimental group). Before transplantation cells were
labeled by adenovirus expressing green fluorescent protein.
Our results showed that it was possible to detect 2 types of
donor cells in the recipient liver of control and experimental
groups: 1) hepatocyte-like cells in liver parenchyma; 2) small,
spindle-shaped, rounded and triangular cells in liver sinusoids
and portal areas. Transplantation after partial hepatectomy
leads to significant increase of transplanted cells homing and
stimulation of their differentiation into hepatocytes. Over
the whole experiment there was no hepatic stellate cells
transdifferentiation into myofibroblasts, thus there is no risk of
liver fibrosis development after this cell type transplantation.
In summary hepatic stellate cells after being transplanted are
able to differentiate into hepatocytes and do not induce liver
fibrosis, that confirms their role in organ regeneration and
probable belonging to hepatic progenitor cells.
About the authors
A K Shafigullina
Kazan State Medical University, KazanKazan State Medical University, Kazan
A A Gumerova
Kazan State Medical University, KazanKazan State Medical University, Kazan
A A Trondin
Kazan State Medical University, KazanKazan State Medical University, Kazan
M A Titova
Kazan State Medical University, KazanKazan State Medical University, Kazan
I M Gazizov
Kazan State Medical University, KazanKazan State Medical University, Kazan
G R Burganova
Kazan State Medical University, KazanKazan State Medical University, Kazan
M S Kaligin
Kazan State Medical University, KazanKazan State Medical University, Kazan
D I Andreeva
Kazan State Medical University, KazanKazan State Medical University, Kazan
A A Rizvanov
Kazan Federal (Volga Region) University, KazanKazan Federal (Volga Region) University, Kazan
A R Muhamedov
Kazan State Medical University, KazanKazan State Medical University, Kazan
A P Kiassov
Kazan State Medical University, KazanKazan State Medical University, Kazan
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