Preparation and reversible aggregation of human cells encased in biocompatible polysaccharide shell

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A deposition of cationic (chitosan) and anionic (alginic
acid) polysaccharides onto the surface of normal and cancer
human cells was studied with the use of dynamic light
scattering. A method for preparation of multilayer polymeric
shell by means of electrostatic adsorption of polysaccharides
onto cell plasma membrane has been proposed. According to
confocal microscopy, the polymeric shell evenly covers the cell
and is 1-5 μm in thickness. Under experimental conditions,
the modification with polysaccharides inhibits human skin
fibroblasts growth but does not exhibit cytotoxicity to
HeLa cells. We developed an approach to controllable and
reversible aggregation of modified cells by their cross-linking
in the presence of calcium ion. Resulting aggregates have
spherical shape and the size of 100-500 μm. Proposed
approaches and methods represent an alternative to cell
microencapsulation technique and are of interest for the
development of three-dimensional cell models and their
delivery in vivo.

About the authors

R A Mukhamadeeva,

Kazan (Volga Region) Federal University, Kazan

Kazan (Volga Region) Federal University, Kazan

I I Mavlyutova,

Kazan (Volga Region) Federal University, Kazan

Kazan (Volga Region) Federal University, Kazan

O V Bondar

Kazan (Volga Region) Federal University, Kazan

Kazan (Volga Region) Federal University, Kazan


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