Efficiency and mechanism of antitumor activity of cardiolipin-like lipid/thymidine kinase gene HSV-tklipoplexes in the presence of gancyclovir
- Authors: Moskovtsev AA1, Sokolovskaya AA1, Ibragimova MY.2, Dojnikova AN2, Sebyakin UL3, Zhdanov RI1,2,4
-
Affiliations:
- SRI General Pathology and Pathophysiology of RAMS, Moscow
- Кazan (Volga region) Federal University, Kazan
- Moscow State University of Fine Chemical Technology, Moscow
- Issue: Vol 7, No 3 (2012)
- Pages: 116-120
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121633
- DOI: https://doi.org/10.23868/gc121633
- ID: 121633
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Abstract
The herpes simplex virus thymidine kinase/gancyclovir
(HSV-tk/GCV) system is studied as cytotoxic lipoplex based
on cardiolipin-like dicationic lipid CDL-I. It is proposed to be
used as nonviral gene transfer system in cancer gene therapy
protocols. Аn efficient transfection of MCF7 and HEC293 cell
lines with this lipoplex was earlier demonstrated. Non-viral
system based on the CDL-I/HSV-tk lipoplex and ganciclovir
treatment causes efficiently death of tumor cells with an
involvement of apoptosis key stages. It was proved that
depolarization of mitochondrial membrane and increased level
of NF-kB transcription factor take place as a response to CDL-I
/ HSV-tk lipoplex action followed by gancyclovir treatment. It
suggests an early involvement of apoptosis mitochondrial way
to an action of this certain «suicide» system, delivered using
dicationic lipid.
(HSV-tk/GCV) system is studied as cytotoxic lipoplex based
on cardiolipin-like dicationic lipid CDL-I. It is proposed to be
used as nonviral gene transfer system in cancer gene therapy
protocols. Аn efficient transfection of MCF7 and HEC293 cell
lines with this lipoplex was earlier demonstrated. Non-viral
system based on the CDL-I/HSV-tk lipoplex and ganciclovir
treatment causes efficiently death of tumor cells with an
involvement of apoptosis key stages. It was proved that
depolarization of mitochondrial membrane and increased level
of NF-kB transcription factor take place as a response to CDL-I
/ HSV-tk lipoplex action followed by gancyclovir treatment. It
suggests an early involvement of apoptosis mitochondrial way
to an action of this certain «suicide» system, delivered using
dicationic lipid.
About the authors
A A Moskovtsev
SRI General Pathology and Pathophysiology of RAMS, MoscowSRI General Pathology and Pathophysiology of RAMS, Moscow
A A Sokolovskaya
SRI General Pathology and Pathophysiology of RAMS, MoscowSRI General Pathology and Pathophysiology of RAMS, Moscow
M Ya Ibragimova
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
A N Dojnikova
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
U L Sebyakin
Moscow State University of Fine Chemical Technology, MoscowMoscow State University of Fine Chemical Technology, Moscow
R I Zhdanov
SRI General Pathology and Pathophysiology of RAMS, Moscow;Кazan (Volga region) Federal University, Kazan;SRI General Pathology and Pathophysiology of RAMS, Moscow;Кazan (Volga region) Federal University, Kazan;
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