Efficiency and mechanism of antitumor activity of cardiolipin-like lipid/thymidine kinase gene HSV-tklipoplexes in the presence of gancyclovir

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Abstract

The herpes simplex virus thymidine kinase/gancyclovir
(HSV-tk/GCV) system is studied as cytotoxic lipoplex based
on cardiolipin-like dicationic lipid CDL-I. It is proposed to be
used as nonviral gene transfer system in cancer gene therapy
protocols. Аn efficient transfection of MCF7 and HEC293 cell
lines with this lipoplex was earlier demonstrated. Non-viral
system based on the CDL-I/HSV-tk lipoplex and ganciclovir
treatment causes efficiently death of tumor cells with an
involvement of apoptosis key stages. It was proved that
depolarization of mitochondrial membrane and increased level
of NF-kB transcription factor take place as a response to CDL-I
/ HSV-tk lipoplex action followed by gancyclovir treatment. It
suggests an early involvement of apoptosis mitochondrial way
to an action of this certain «suicide» system, delivered using
dicationic lipid.

About the authors

A A Moskovtsev

SRI General Pathology and Pathophysiology of RAMS, Moscow

SRI General Pathology and Pathophysiology of RAMS, Moscow

A A Sokolovskaya

SRI General Pathology and Pathophysiology of RAMS, Moscow

SRI General Pathology and Pathophysiology of RAMS, Moscow

M Ya Ibragimova

Кazan (Volga region) Federal University, Kazan

Кazan (Volga region) Federal University, Kazan

A N Dojnikova

Кazan (Volga region) Federal University, Kazan

Кazan (Volga region) Federal University, Kazan

U L Sebyakin

Moscow State University of Fine Chemical Technology, Moscow

Moscow State University of Fine Chemical Technology, Moscow

R I Zhdanov

SRI General Pathology and Pathophysiology of RAMS, Moscow;Кazan (Volga region) Federal University, Kazan;

SRI General Pathology and Pathophysiology of RAMS, Moscow;Кazan (Volga region) Federal University, Kazan;

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