Uluchshenie dostavki kompleksov plazmidnoy DNKs polikationom v kletki chelovekav prisutstvii bloksopolimerov etilen-i propilenoksida

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access


The work is aimed at the study of the effect of novel
block copolymers of ethylene oxide and propylene oxide
on the delivery of plasmid DNA and its complexes with
cationic polymers into human cells. Tri-functional amphiphilic
block copolymers on the basis of glycerol (LaprolsTM),
polyethyleneimine (25 kDa) and commercial transfection
reagent TurboFectTM were tested as delivery systems.
TurboFect was found to form more compact and positively
charged polyplexes with plasmid DNA (pEGFP-N2) and
provided higher expression of GFP in HEK 293 cells
compared to polyethyleneimine. Laprols weakly interacted
with plasmid DNA and did not improve its intracellular
delivery. However they markedly promoted cell transfection
by DNA-polyethyleneimine complexes. Results show that
Laprols exhibit mild cytotoxicity and produce the interest for
gene therapeutics delivery into cells.

About the authors

A D Martynova,

Kazan (Volga Region) Federal University, Kazan

Kazan (Volga Region) Federal University, Kazan

V D Shevchenko,

Kazan (Volga Region) Federal University, Kazan

Kazan (Volga Region) Federal University, Kazan

O V Bondar,

Kazan (Volga Region) Federal University, Kazan

Kazan (Volga Region) Federal University, Kazan

T I Abdullin

Kazan (Volga Region) Federal University, Kazan

Kazan (Volga Region) Federal University, Kazan


  1. Kay M.A., Glorioso J.C., Naldini L. Viral vectors for gene therapy: the art of turning infectious agents into vehicles of therapeutics. Nature Medicine 2001; 7(I): 33-40.
  2. Thomas M., Klibanov A.M. Non-viral gene therapy: polycationmediated DNA delivery. Appl. Microbiol. Biotechnol. 2003; 62: 27-34.
  3. Tiera M.J., Shi Q., Winnik F.M. et al. Polycation-based gene therapy: current knowledge and new perspectives. Current Gene Therapy 2011; 11(IV): 288-306.
  4. Patil S.D., Rhodes D.G., Burgess D.J. DNA-based therapeutics and DNA delivery systems: a comprehensive review. AAPS 2005; 7(1): 61-77.
  5. Petersen H., Kunath K., Martin A.L. et al. Star-shaped poly(ethylene glycol)-blockpolyethylenimine copolymers enhance DNA condensation of low molecular weight polyethylenimines. Biomacromolecules 2002; 3(V): 926-36.
  6. Lemkine G.F., Demeneix B.A. Polyethylenimines for in vivo gene delivery. Curr. Opin. Mol. Ther. 2001; 3: 178-82.
  7. Boussif O., Lezoualc'h F., Zanta M.A. et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. PNAS USA 1995; 92(XVI): 7297-301.
  8. Batrakova E.V., Kabanov A.V. Pluronic block copolymers: evolution of drug delivery concept from inert nanocarriers to biological response modifiers. Control Release 2008; 130: 98-106.
  9. Astafieva I., Maksimova I., Lukanidin E. et al. Enhancement of the polycation-mediated DNA uptake and cell transfection with Pluronic P85 block copolymer. FEBS letters 1996; 389(III): 278-80.
  10. Блатт Н.Л., Ялвач М.Э., Шафигуллина А.К. и др. Влия- ние Плуроника Р85 на пролиферацию и остеогенную диффе- ренцировку мезенхимных стволовых клеток человека in vitro. Клеточная Трансплантология и Тканевая Инженерия 2010; 5(III): 66-70.
  11. Feldman L.J., Pastore C.J., Aubailly N. et al. Improved efficiency of arterial gene transfer by use of poloxamer 407 as a vehicle for adenoviral vectors. Gene Ther. 1997; 4(III): 189-98.
  12. Dishart K.L., Denby L., George S.J. et al. Third-generation lentivirus vectors efficiently transduce and phenotypically modify vascular cells: implications for gene therapy. J. Mol. Cell. Cardiol. 2003; 35(VII): 739-48.
  13. Lemieux P., Guerin N., Paradis G. et al. A combination of poloxamers increases gene expression of plasmid DNA in skeletal muscle. Gene Therapy 2000; 7: 986-91.
  14. Yang Z., Zhu J., Sriadibhatla S. et al. Promoter- and strainselective enhancement of gene expression in a mouse skeletal muscle by a polymer excipient Pluronic P85. Control. Release. 2005; 108: 496-512.
  15. Штырлин Ю.Г., Абдуллин Т.И., Бондарь О.В. и др. Трехфунк- циональные блоксополимеры этиленоксида и пропиленоксида для доставки активных веществ в живые клетки. Заявка на патент РФ №2012119390. 2012 Май 11.

Copyright (c) 2012 Eco-Vector

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС 77 - 85657 от 21.07.2023 от 11.03.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies