Human umbilical cord blood mononuclear cells transfected with dual cassette plasmids(vegf + neurotrophic factor) for the treatment of amyotrophic lateral sclerosis
- Authors: Kudryashova NV1, Guseva DS2, Salafutdinov II1, Bashirov FV1, Kiiasov AP1, Rizvanov AA1, Islamov RR2
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Affiliations:
- Кazan (Volga region) Federal University, Kazan
- Кazan State Medical University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 92-97
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121614
- DOI: https://doi.org/10.23868/gc121614
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Full Text
Abstract
diseases, after neurotraumas and ischemic strokes the
most important neurotrophic and neuroprotective factors,
which can be used as therapeutic agents were identified
in long-term studies in vitro and in vivo. These include
brain-derived neurotrophic factor (BDNF), glial-derived
neurotrophic factor (GDNF), insulin-like growth factor (IGF)
and vascular endothelial growth factor (VEGF). One of the
promising ways of the delivery of supporting neuron survival
factors is considered to be transplantation of genetically
modified cells overexpressing recombinant therapeutic
genes. This article describes generation of cellular delivery
vectors of therapeutic genes - human umbilical cord blood
mononuclear cells genetically modified by dual cassette
plasmids, expressing two therapeutic genes. Efficiency of
transgene expression was confirmed in vitro using RT-PCR.
Analysis of survival, migration, and phenotype of genetically
modified cells was performed 2 weeks after transplantation
into transgenic mice with amyotrophic lateral sclerosis
phenotype.
About the authors
N V Kudryashova
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
D S Guseva
Кazan State Medical University, KazanКazan State Medical University, Kazan
I I Salafutdinov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
F V Bashirov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
A P Kiiasov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
A A Rizvanov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
R R Islamov
Кazan State Medical University, KazanКazan State Medical University, Kazan
References
- Ризванов А.А., Гусева Д.С., Салафутдинов И.И. и др. Докли- нические исследования терапии бокового амиотрофического скле- роза с помощью генетически модифицированных мононуклеарных клеток крови пуповины. Клеточная Трансплантология и Тканевая Инженерия 2012; 7(2): 56-63.
- Исламов Р.Р., Ризванов А.А., Киясов А.П. Боковой амиотро- фический склероз: стратегия генно-клеточной терапи. Неврологи- ческий вестник 2008; 40(4): 91-101.
- Rizvanov A.A., Guseva D.S., Salafutdinov I.I. et al., Genetically modified human umbilical cord blood cells expressing vascular endothelial growth factor and fibroblast growth factor 2 differentiate into glial cells after transplantation into amyotrophic lateral sclerosis transgenic mice. Exp. Biol. Med. 2011; 236(1): 91-8.
- Салафутдинов И.И., Шафигуллина А.К., Ялвач M.Э. и др. Эффект одновременной экспрессии различных изоформ фактора роста эндотелия сосудов VEGF и основного фактора роста фибро- бластов FGF2 на пролиферацию эндотелиальных клеток пупочной вены человека HUVEC. Клеточная Трансплантология и Тканевая Ин- женерия 2010; 5(2): 62-7.
- Ризванов А.А., Гусева Д.С., Салафутдинов И.И. и др. Генно- клеточная терапия бокового амиотрофического склероза монону- клеарными клетками пуповинной крови человека, трансфицирован- ными генами нейронной молекулы адгезии L1CAM и сосудистого эндотелиального фактора роста VEGF. Клеточная трансплантология и тканевая инженерия 2010; 5(4): 55-65.
- Szulc J., Wiznerowicz M., Sauvain M.O. et al. A versatile tool for conditional gene expression and knockdown. Nat. Methods. 2006; 3(2): 109-16.
- Hawley T.S., Hawley R.G., Totowa N.J. Flow Cytometry Protocols 2ed. Methods in Molecular Biology: Humana Press; 2004: 424.
- Rizvanov A.A., Kiyasov A.P., Gaziziov I.M. et al. Human umbilical cord blood cells transfected with VEGF and L(1)CAM do not differentiate into neurons but transform into vascular endothelial cells and secrete neuro-trophic factors to support neuro-genesis-a novel approach in stem cell therapy. Neurochem. Int. 2008; 53(6): 389-94.
- Chintalgattu V., Nair D.M., Katwa L.C. Cardiac myofibroblasts: a novel source of vascular endothelial growth factor (VEGF) and its receptors Flt-1 and KDR. J. Mol. Cell Cardiol. 2003; 35(3): 277-86.
- Jiao Y., Sun Z., Lee T. et al. A simple and sensitive antigen retrieval method for free-floating and slide-mounted tissue sections.
- J. Neurosci. Methods. 1999; 93(2): 149-62.
- Lunn J.S., Hefferan M.P., Marsala M. et al. Stem cells: comprehensive treatments for amyotrophic lateral sclerosis in conjunction with growth factor delivery. Growth Factors 2009; 27(3): 133-40.
- Hester M.E., Foust K.D., Kaspar R.W. et al. AAV as a gene transfer vector for the treatment of neurological disorders: novel treatment thoughts for ALS. Curr. Gene Ther. 2009; 9(5): 428-33.
