The study of migration of the multipotent mesenchymal stromal cells in the body of animal with a tumor

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Abstract

The study of stem and progenitor cells role in carcinogenesis is the subject of intense investigation. At the same time fluorescent methods become more common for the studying of the stem cells migration into recipient organism and their involvement in tumor pathogenesis. In this study the interaction between bone marrow multipotent mesenchymal stromal cells (MMSC) and allogenic tumor (Lewis lung carcinoma) was investigated using the methods of the flow cytometry and a confocal laser scanning microscopy. MMSC were isolated from bone marrow of the males GFP+-transgenic mice C57/Bl6. The tumors were implanted in mice C57/Bl6 by subcutaneous injection of Lewis lung carcinoma tumor cells. The day before that myeloablation was performed by irradiation. GFP+-MMSC and bone marrow cells from the males C57/Bl6 were administrated into mice C57/Bl6 systemically on the following day after irradiation. The animals were divided into two groups: 1 — irradiated mice with tumor inoculation and injection of bone marrow cells including GFP+-MMSC; 2 (control) — unirradiated mice with tumor inoculation but without cells transplantation. The fluorescence level of freshly isolated bone marrow cells from animals of the first and control groups was evaluated by flow cytometry on the 7, 12 and 15 days after GFP+-MMSC transplantation. In these groups of animals we analyzed the distribution of the labeled GFP+-MMSC and their descendants in the organs and tumor tissues by laser scanning microscopy also on the 7, 12 and 15 days after transplantation. It was established that in systemic administration donor GFP+-MMSC were absent in the bone marrow and tissues of induced tumors, but at the same time they were able to migrate into recipients accumulating in the potential niches (spleen, liver) and proliferate actively.

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A. V Meleshina

Nizhny Novgorod State University, Nizhny Novgorod

E. I Cherkasov

Nizhny Novgorod State University, Nizhny Novgorod

E. A Sergeeva

Institute of Applied Physics of Russian Academy of Science, Nizhny Novgorod

M. V Shirmanova

Nizhny Novgorod State Medical Academy, Nizhny Novgorod

I. V Balalaeva

Institute of Applied Physics of Russian Academy of Science, Nizhny Novgorod

E. V Kiseleva

N.K. Koltzov Institute of Developmental Biology of RAS, Moscow

E. V Zayganova

Nizhny Novgorod State Medical Academy, Nizhny Novgorod

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