Comparison of different methods for generation of functional human cardiomyocytes

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Abstract

Generation of human cardiomyocytes in vitro is important for basic science and for regenerative medicine. There are a lot of published protocols describing the cardiomyocytes obtaining with different efficacy. However in practice, a researcher is faced with the fact that published methods have to be adapted to a particular laboratory for specific tasks. The aim of this study was to compare three methods for generation of human cardiomyocytes in vitro: 1) from human adult heart sample by purifying myocardial progenitor cells and their subsequent differentiation into cardiomyocyte direction; 2) from human adipose tissue multipotent mesenchimal stromal cells (AT-MSC); 3) from human dermal fibroblasts using reprogramming technology and subsequent differentiation to cardiomyocytes. We have shown relatively low efficiency of cardiac progenitor cells and AT-MSC capacity to give rise to cardiomyocyte lineage. The effectiveness of cardiomyocytes differentiation from dermal fibroblasts by reprogramming technology was significantly higher. The advantages, disadvantages and perspectives of each method are discussed.

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About the authors

A. A Khudiakov

Federal Almazov heart blood and endocrinology centre, Saint-Petersburg

D. I Kurapeev

Federal Almazov heart blood and endocrinology centre, Saint-Petersburg

A. A Kostareva

Federal Almazov heart blood and endocrinology centre, Saint-Petersburg

A. B Malashicheva

Federal Almazov heart blood and endocrinology centre, Saint-Petersburg

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