Cryopreserved multipotent mesenchymal stromal cells stimulate reparative chondrogenesis in degenerated intervertebral disc
- Authors: Iukhta M.S1, Volkova N.A1, Zhulikova E.P1, Goncharuk E.I1
-
Affiliations:
- Institute for Problems of Cryobiology and Cryomedicine of Ukraine NAS, Kharkov, Ukraine
- Issue: Vol 8, No 2 (2013)
- Pages: 29-34
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121598
- DOI: https://doi.org/10.23868/gc121598
Cite item
Abstract
The choice object for cell therapy of degenerative changes of the intervertebral discs is a cryopreserved multipotent mesenchymal stromal cells (MMSC). The research aim was to evaluate a therapeutic potential of cryopreserved allogenic marrow-derived MMSC in this pathology. Rats with modeled compressive degenerative damage of the intervertebral disc CcVI-VII were introduced by 0.5x10 B native or cryopreserved as well as PKH-26-labeled cells on a collagen sponge in the defect area. Animals with spontaneous recovery and administration of saline were served as a control. Histomorphometric study and fluorescent microscopy of intervertebral disc sections was performed on the 30th day after treatment. There was extremely low regenerative potential in the control groups of animals. Histologically after cell therapy, there was tendency to repair of cracks, fissures, collagen fiber fragmentations of the fibrous ring, which was more pronounced in the case of the native MMSC culture application. The cryopreserved MMSC administration was accompanied by increasing in the fibrochondrocyte number of the dorsal annulus per unit area of the intervertebral disc CcVI-VII slice at 1.25 times relative to the model values. At the same time the fibrous ring height increased by 12.5±3.3%, and densitometric index of cartilaginous tissue — by 64±5.7% relative to the model values. The luminescence in the red range of the spectrum in the form of drops and conglomerates, which was localized in outer parts of fibrous ring, was detected by fluorescent microscopy of intervertebral disc cryostat sections on the 30 day after introduction of labeled native or cryopreserved MMSC, that indicated the safety and partial migration of transplanted and/ or their daughter cells in the intervertebral disc CcVI-VII. Our data showed a stimulating effect of native and cryopreserved marrow-derived MMSC suspension in degenerative intervertebral disc damages.
Full Text

About the authors
MS. S Iukhta
Institute for Problems of Cryobiology and Cryomedicine of Ukraine NAS, Kharkov, Ukraine
N. A Volkova
Institute for Problems of Cryobiology and Cryomedicine of Ukraine NAS, Kharkov, Ukraine
E. P Zhulikova
Institute for Problems of Cryobiology and Cryomedicine of Ukraine NAS, Kharkov, Ukraine
E. I Goncharuk
Institute for Problems of Cryobiology and Cryomedicine of Ukraine NAS, Kharkov, Ukraine
References
- Richardson S., Walker R., Parker S. et al. Differentiation of human mesenchymal stem cells to nucleus pulposus cells using a novel co-culture technique. Euro. Cells Mat. 2005; 10 tII):56.
- Mamidi M.K., Nathan K.G., Singh G. et al. Comparative cellular and molecular analyses of pooled bone marrow multipotent mesenchymal stromal cells during continues passaging and after successive cryopreservation. J. Cell Biochem. 2012; 113: 3153-64.
- Адамс Р. Методы культуры клеток для биохимиков. М.: Мир; 1983.
- Pazzaglia U.E., Andrini L., Di Nucci A. The effects of mechanical forces on bones and joints. Experimental study on the rat tail. J. Bone Joint Surg. Br. 1997; 79: 1024-30.
- Wallace P.K., Muirhead K.A. Cell tracking 2007: a proliferation of probes and applications. Immunol. Invest. 2007; 36:527-61.
- Волкова О.В., Елецкий Ю.К. Основы гистологии с гистологической техникой. М.: Медицина; 1982.
- Автандилов Г.Г. Медицинская морфометрия. Руководство. М.: Медицина; 1990.
- Council of Europe [France]. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg,18.III.1986, http://conventions.coe. int/treaty/en/Treaties/Word/123.doc.
- Ирьянов Ю.М., Силантьева Т.А. Современные представления о гистологических аспектах репаративной регенерации костной ткани [обзор литературы). Клеточные источники репаративного остеогенеза. Гетерогенность клеточной популяции в области травматического повреждения кости. Гений ортопедии 2007; 2:111-16.
- Ho G., Leung V.Y.L., Cheung K.M. C. Effect of severity of intervertebral disc injury on mesenchymal stem cell-based regeneration. Connect. Tiss. Res. 2008; 49:15-21.
- Michalek A.J., Buckley M.R., Bonassar L.J. et al. The effects of needle puncture injury on microscale shear strain in the intervertebral disc annulus fibrosus. The Spine J. 2010; 10: 1098-105.
- Salazar K.D., Lankford S.M., Brody A.R. Mesenchymal stem cells produce Wnt isoforms and TGFb1 that mediate proliferation and procollagen expression by lung fibroblasts. Am. J. Physiol. Lung Cell Mol. Physiol. 2009; 297: L1002-11.
- Togel F., Hu Z., Weiss K. et al. Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms. Am. J. Physiol. Renal. Physiol. 2005; 289: F31-42.
- Hiyama A., Mochida J., Iwashina T. Transplantation of mesenchymal stem cells in a canine disc degeneration model. J. Orthop. Res. 2008; 26(5):589-600.
